摘要
利用前期通过大量预试验筛选获得的3种真菌多糖激发子,在桑黄Sanghuangporus sanghuang发酵液中进行添加,研究其对桑黄胞内黄酮、三萜及多糖等代谢产物积累的影响,并对激发效果好的激发子继续进行添加量和激发时间的优化。结果显示:参试的3种真菌多糖激发子在一定程度上均能促进桑黄胞内黄酮和胞内三萜类成分的积累,但对胞内多糖积累的影响不明显;来源菌株为薄多年卧孔菌Perenniporia tenuis的激发子表现最突出,激发效果显著且稳定性良好。激发条件优化的结果表明:在接种的同时按照50mg/L的终浓度进行激发子的添加,激发6d,桑黄胞内黄酮含量达最高值0.7%,比对照提高79.49%;在接种1d后,按照50mg/L的终浓度进行激发子的添加,激发5d,对桑黄胞内三萜类成分代谢的激发效果最显著,胞内三萜含量最高可达5.62%,是对照组的3.43倍。
The effects of fungal elicitor on the accumulation of Sanghuongporus sanghuang intracellular metabolites, such as flavonoids, triterpenoid and polysaccharide, were investigated. Three fungal polysaccharide elicitors were obtained through previous preliminary experiments. The optimal additive amount and stimulation time were optimized by using the best elicitor The results indicated that the activated effects of the three fungal elicitors on the accumulation of 5. sanghuang intracellular flavonoids and triterpenoid were notable, but the effects on the accumulation of intracellular polysaccharide were indistinctiveThe elicitor obtained from Perenniporia tenuis mycelia in submerged fermentation owned good excitation effect and stability After excitation condition optimization the intracellular flavonoid content was 79.49% higher as compared with the control, reaching 0.7% in the sixth day of cultivation under final concentration of 50mg/L and six-day elicitor stimulation. The highest ntracellular triterpenoid content was up to 5.62% and became 3.43 times of the control under the final concentration of 50mg/L and five-day elicitor stimulation
出处
《菌物学报》
CAS
CSCD
北大核心
2017年第4期482-491,共10页
Mycosystema
基金
国家自然科学基金(31301827
31572181)
山东省自然科学基金(ZR2012CL03)
山东省重点研发计划项目(2016GGB01663)
青岛农业大学高层次人才基金(631445)~~
关键词
桑黄
真菌激发子
多糖
黄酮
三萜
Sanghuangporus sanghuang, fungal elicitor, polysaccharide, flavonoid, triterpenoid
