摘要
为研发高效低毒的苹果树腐烂病生防制剂,采用菌丝生长速率法分别研究了不同表面活性剂、防腐剂、紫外保护剂和载体在不同浓度下对解淀粉芽孢杆菌TS-1203菌株生长及其抑菌活性的影响。助剂筛选结果表明:TS-1203的最适表面活性剂为吐温-80,其质量分数为0.50%、培养48h时,对苹果树腐烂病菌的抑制率为88.90%,与对照无显著差异;最适防腐剂为柠檬酸三钠,其质量分数为0.10%、培养48h时,对苹果树腐烂病菌的抑制率为93.39%,显著高于对照;最适紫外保护剂为木质素磺酸钠,其质量分数为0.50%、培养48h时,对苹果树腐烂病菌的抑制率为84.18%,显著高于对照。采用响应面法对TS-1203生防制剂的最佳助剂条件进行了优化,得其最佳配比为:65mL发酵液中分别加入650μL吐温-80、164.58 mg柠檬酸钠及308.12 mg木质素磺酸钠,在此条件下,TS-1203菌剂对苹果树腐烂病菌的抑制率最高,达84.78%。载体筛选结果表明,硅藻土对TS-1203菌株生长促进作用最强,但易分层;海藻酸钠对该菌株生长抑制作用最强;有机膨润土为最佳载体。
In order to develop highly effective and less toxic bio-control agent for the control of apple tree canker in vitro, the effect of surfactants, preservatives, UV-protectors and carriers on the growing strains of Bacillus amyloliquefaciens TS-1203, and the antibacterial activity of the B. amyloliquefaciens TS-1203 against Valsa mali were determined by the method of mycelium growth rate and response surface methodology（RSM）. The results showed that Tween-80 was the most favorable surfactant for the bio-control agent at 0.50% mass fraction. Under this condition, the inhibition rate of TS-1203 was 88.90% at 48 h, which was not significantly different with that of the control. The sodium citrate was the most favorable preservative for the bio-control agent at 0.10% mass fraction. Under this condition, the highest inhibition rate of TS-1203 was 93.39% at 48 h, which was significantly higher than that of the control. Moreover, the sodium lignosulphonate was the most favorable ultraviolet protectant for the bio-control agent at 0.10% mass fraction. Under this condition, the inhibition rate of TS-1203 was 84.18% at 48 h, which was significantly higher than that of the control. The optimum adjuvant for the bio-control agent of TS-1203 was optimized by RSM, and the optimum adjuvant for 65 mL bacterial fermentation contained 650 μL of tween-80, 164.58 mg of sodium citrate and 308.12 mg of sodium lignosulfonate. The inhibitory rate of TS-1203 formulation against V. mali as high as 84.78%, under these conditions. In addition, the diatomite had the strongest effect on the growth of TS-1203, but it was easy to be layered. Sodium alginate had the strongest inhibitory effect on the growth of TS-1203. Therefore, the optimum carrier for bio-control agent TS-1203 was organic bentonite.
作者
孙朝华
侯宝宏
张树武
薛应钰
张文军
徐秉良
SUN Chaohua HOU Baohong ZHANG Shuwu XUE Yingyu ZHANG Wenjun XU Bingliang(College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Chin)
出处
《农药学学报》
CAS
CSCD
北大核心
2017年第2期254-265,共12页
Chinese Journal of Pesticide Science
基金
公益性行业(农业)科研专项(201203034)
国家苹果产业技术体系(CARS-28)
苹果主要病害生物防控技术研发与集成(2016YFD0201112)资助项目
