摘要
目的:探讨黄芪注射液对糖尿病肾病大鼠肾组织脂联素的表达及对糖尿病肾病防治的作用。方法:SD大鼠32只,适应性喂养1周后,随机除外8只大鼠(正常组),余下24只作为糖尿病肾病模型组(DN模型组),对DN模型组24只大鼠按55 mg/kg进行腹腔注射链脲佐菌素(STZ)一次,正常组予等体积的枸橼酸-枸橼酸钠缓冲液腹腔注射。造模成功后,随机分为3组,即模型对照组(模型组)、黄芪注射液组(黄芪组)、氯沙坦治疗对照组(氯沙坦组)。各组给与对相应药物8周后,常规检测体重、血糖、肾功能、24 h尿蛋白,定量PCR检测肾组织脂联素m RA表达,Western Blot检测脂联素蛋白表达。结果:(1)与正常组比较,模型组、黄芪组与氯沙坦组大鼠的血糖、血尿素氮及24 h尿蛋白定量显著增加,体重显著降低,差异具有统计学意义(P<0.05)。与模型组比较,黄芪组、氯沙坦组大鼠血糖及24 h尿蛋白定量显著降低,差异具有统计学意义(P<0.05)。与氯沙坦组比较,黄芪组血糖、血清肌酐、血尿素氮水平及24 h尿蛋白定量差异无统计学意义(P>0.05)。(2)与正常组比较,模型组、黄芪组与氯沙坦组Adiponectin蛋白及m RNA表达明显增加,差异具有统计学意义(P<0.05);与模型组比较,黄芪组、氯沙坦组Adiponectin蛋白及m RNA表达明显降低,差异具有统计学意义(P<0.05);与氯沙坦组比较,黄芪组Adiponectin蛋白及m RNA表达差异无统计学意义(P>0.05)。结论:黄芪注射液确能通过下调肾组织脂联素蛋白及m RNA表达,降低血糖,减少尿蛋白,保护肾功能,从而改善糖尿病肾病。
Objective ： To investigate the effect of Astragalus Injection on the expression of adiponectin in renal tissue of dia- betic nephropathy rats and its effect on prevention and treatment of diabetic nephropathy. Methods：Thirty - two SD rats, adaptive feeding for 1 weeks, randomly except for 8 rats （ normal group） , and the remaining 24 as the diabetic nephropathy model group （ DN model group）. The DN model group 24 rats were received streptozotocin（STZ） by intraperitoneal injection at the dose of 55 mg/kg, and normal group were treated with the same volume of citrate - citric acid sodium buffer by intraperitoneal injection. After successful modeling, The DN model group 24 rats were divided randomly into three groups, model control group （model group） , astragalus in- jection group（ Astragali group） , losartan treatment control group （Losartan group）. Each group was given the corresponding drugs for 8 weeks, routine detection of body weight, blood glucose, serum renal function, 24 hour urinary protein, quantitative PCR was used to detect the expression of Adiponectin mRA in renal tissue, and Blot Western was used to detect the expression of Adiponectiu. Re- suits ： （ 1 ） Compared with the normal group, the rats blood glucose, blood urea nitrogen and 24 hours urinary protein of model group, Astragalus group and the Losartan group was significantly increased, a significant reduction in body weight （ P 〈 0.05 ） , the difference has statistical significance. Compared with the model group, the rats blood glucose and 24 hour urinary protein of Astragalus group, losartan group was significantly reduced （ P 〈 0.05 ）, the difference has statistical significance. Compared with losartan group, the levels of blood glucose, serum creatinine, blood urea nitrogen levels and 24 - hour urinary protein of Astragalus group were no differ- ence （ P 〉 0.05 ） , the difference is not statistically significant. （2） Compared with the normal group, the renal tissue Adiponectin pro- tein and mRNA expression of model group ,Astragalus group and losartan group were significantly increased （ P 〈 0.05 ） , the differ- ence has statistical significance; the model group compared with Astragalus group and losartan group, Adiponectin protein and mRNA expression decreased significantly （ P 〈 0.05 ）, the difference has statistical significance ; the losartan group compared with Astragalus group, Adiponectin mRNA and protein expression bad no obvious difference （ P 〉 0.05 ） , the difference is not statistically significant. Condusion：Astragalus injection can reduce blood glucose, urinary protein and protect renal function by reducing the expression of Adiponectin protein and mRNA in renal tissue, thereby it can improve diabetic nephropathy.
出处
《中国中西医结合肾病杂志》
2017年第2期106-109,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
浙江省大学生科技创新活动计划暨新苗人才计划项目(No.2015R410001)
关键词
黄芪注射液
糖尿病肾病
脂联素
实验研究
Astragalus injection Diabetic Nephropathy Adiponectin experimental study
