摘要
为了解广西H6亚型AIV感染情况,本研究于2013~2015年间随机采集了南宁、柳州、北海、防城港、梧州五市活禽市场中的鸡棉拭子、鸭棉拭子及环境棉拭子并用PCR对棉拭子进行检测,分析结果表明,广西H6亚型AIV感染的阳性率为5.93%(736/12 415),且呈逐年上升趋势;鸭棉拭子感染率6.91%(434/6 280)高于鸡棉拭子感染率4.06%(203/4 995);在所有采集的棉拭子中,共分离获得H6亚型AIV毒株9株,分离率为0.08%。另外,受气候影响,春、冬两季感染率较高,且南宁市感染率高于其他四市。本次流行病学调查结果能较好地评估目前广西H6亚型AIV流行情况,为进一步做好H6亚型AIV感染防控工作提供了重要实验依据。
In order to understand the situation of H6 subtype AIV infection in Guangxi, we randomly collected the chicken cotton swabs, duck cotton swabs and environmental cotton swabs from the live poultry market in Nanning, Liuzhou, Beihai, Fangchenggang, and Wuzhou cities in 2013-2015, and employed the PCR detection the swabs. The analysis results showed that the positive rate of H6 subtype AIV infection in Guangxi was 5.93% (736/12 415) with a tendency of rising year by year. The duck cotton swab infection rate was 6.91% (434/6 280), which was higher than that of the chicken cotton swab infection rate of 4.06% (203/4 995). We totally isolated 9 H6 subtype AIV strains, and the isolation rate was 0.08%. In addition, the infection rate was higher in spring and winter affected by the climate and infection rate in Nanning was higher than the other four cities. The results of epidemiological investigation could display a good evaluation of H6 subtype influenza virus infection in Guangxi, which would provide an important experimental data for prevention and controlling the H6 subtype AIV infection.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第2期589-594,共6页
Genomics and Applied Biology
基金
广西H6亚型禽流感病毒分离
鉴定和基因序列分析(桂渔牧科1304544)基金项目
2012ZX10004214-00-1-003基金项目共同资助
关键词
H6亚型
禽流感
流行病学调查
棉拭子
H6 subtype, Avian influenza virus (AW), Epidemiological investigation, Cotton swabs