摘要
目的建立血细胞参数个性化参考区间并验证。方法随机选取2014、2015、2016连续3年各项检查结果均正常的体检健康者,共2 089例,收集血常规结果。将体检者同一指标的后一次检测结果与前一次的比值定义为波动性(λ)。计算2015年结果与2014年的比值(λ1),对λ1进行统计学分析并建立波动性参考区间(CIλ);计算2016年结果与2015年的比值(λ2),用λ2对CIλ验证。将各体检者2015年血常规结果分别与CIλ的上、下限相乘,得出各体检者的个性化参考区间(CI_p),用2016年的血常规结果对CI_p验证;分别计算CI_p和行业标准参考区间的上、下限比值。结果 CIλ:白细胞计数(0.66~1.53)、淋巴细胞绝对值(0.67~1.51)、单核细胞绝对值(0.50~2.00)、中性粒细胞绝对值(0.56~1.78)、嗜酸性粒细胞绝对值(0.4~2.51)、血小板计数(0.76~1.32)、红细胞计数(0.92~1.12)、血红蛋白(0.92~1.11)、红细胞压积(0.91~1.12)、平均红细胞体积(0.95~1.07)、平均红细胞血红蛋白含量(0.95~1.05)、平均红细胞血红蛋白浓度(0.94~1.06);CIλ的验证试验表明CIλ适合该实验室;CI_p的验证试验表明CI_p适合该实验室;与行业标准参考区间相比,CI_p的上、下限比值更小。结论建立并验证了CI_p;CI_p较传统参考区间更敏感、个性化。
Objective To establish and verify the personalized reference interval of blood cells. Methods The results of blood cells from 2 089 health subjects in 2014,2015 and 2016 were collected. The ratio of the later results to the previous results was defined as the fluctuation（ λ）. The ratio（ λ1） of the results of 2015 to the results of 2014 was calculated and λ1was analyzed statistically to establish the fluctuation reference interval（ CIλ）. The ratio（ λ2） of the results of 2016 to the results of 2015 was calculated. λ2was used to verify λ2. The personalized reference interval（ CI_p） was established by multiplying each result of 2015 and CIλ. CI_pwas verified by results of 2016. The ratio of the upper and lower limits of CI_pwas calculated. The ratio of the upper and lower limits of the reference interval（ WS/T 405） was calculated. Results The values of CIλwere as follows： WBC（ 0. 66 to 1. 53）,L（ 0. 67 to 1. 51）,M（ 0. 50 to 2. 00）,N（ 0. 56 to 1. 78）,E（ 0. 4 to 2. 51）,PLT（ 0. 76 to 1. 32）,RBC（ 0. 92 to 1. 12）,Hb（ 0. 92 to 1. 11）,Hct（ 0. 91 to1. 12）,MCV（ 0. 95 to 1. 07）,MCH（ 0. 95 to 1. 05） and MCHC（ 0. 94 to 1. 06）. The validation tests of CIλand CI_pshowed that both CIλand CI_pwere suitable for this laboratory. Compared with the reference interval of professional criteria,the ratio of the upper and lower limits of the CI_pwas smaller than that of traditional criteria. Conclusion CI_pfor this laboratory was established and verified.Compared with traditional criteria,CI_pshould be more personalized and highly sensitive.
出处
《临床检验杂志》
CAS
CSCD
2017年第2期107-110,共4页
Chinese Journal of Clinical Laboratory Science
基金
上海市卫生和计划生育委员会资助项目(20164Y0246)
关键词
个性化医疗
血细胞
参考区间
波动性
personalized medicine
blood cell
reference interval
fluctuation
