摘要
目的评价Sysmex XN-9000血液分析仪(简称"XN-9000")前体白细胞通道(WPC)检测外周血触发WBC报警的可靠性。方法采用与手工血涂片白细胞形态镜检结果比对的方法,复核520例分别经Sysmex XE-2100血液分析仪(简称"XE-2100")和XN-9000检测触发WBC报警的标本,按报警种类统计判读结果。结果 XN-9000 WPC通道的Blast(原始细胞)报警比XE-2100特异性高(χ~2=6.98,P<0.05),XN9000将假阳性率从XN2100的64%降低至36%(χ~2=6.18,P<0.05)。XN-9000 WPC的Blast/Abn Lympho(原始/异常淋巴细胞)综合有效率90.4%、94.4%,高于XE2100的72.2%和80.1%(χ~2分别为5.6、6.33,P均<0.05)。在WPC通道自动复测模式下,在所有被检标本(体验健康者和患者,包括新生儿)中,WPC通道Blast/Abn Lympho(原始/异常淋巴)报警的特异性和总有效率高于WDF(χ~2=6.15、4.14、6.02、4.88,P<0.05),降低了WDF通道误报率;XN-9000(WDF/WPC)总报警复片率低于XE-2100(χ~2=4.33,P<0.05);而XN-9000(WDF/WPC)Blast/Abn Lympho(原始/异常淋巴)报警的复片率为32%(84/260),低于XE-2100的41%(107/260)(χ~2=4.38,P<0.05)。结论XN-9000 WPC通道相较于XE-2100,提高了Blast/Abn Lympho(原始/异常淋巴)报警的综合有效性,降低了误报率和复片率,提高了临床实验室的工作效率。
Objective To evaluate the reliability of the white precursor cell（ WPC） channel on the Sysmex XN-9000 hematology analyzer（ XN-9000） in giving the abnormal white blood cell（ WBC） alarm. Methods A total of 520 blood samples with abnormal WBC alarm from the Sysmex XE-2100（ XE-2100） and XN-9000 were performed the manual peripheral blood smear review,and their results were analyzed. Results The specificity of the XN-9000 WPC channel in the blast alarm was significantly higher than that of XE-2100（ χ^2= 6. 98,P〈0. 05）,while the false positive rate of the XN-9000（ 36%） significantly lower than that of XE-2100（ 64%,χ^2=6. 18,P〈0. 05）. The overall efficiency of the XN-9000 WPC channel in identifying blasts and abnormal lymphocytes（ 90. 4% and94. 4%） was significantly higher than that of the XE-2100（ 72. 2% and 80. 1%,χ^2= 5. 6 and 6. 33,P〈0. 05）. Under the automatic reflex analysis mode of WPC channel,the specificity and overall efficiency of the WPC channel in identifying blasts and abnormal lymphocytes of all the detected samples were significantly higher than those of the WDF channel（ χ^2= 6. 15,4. 14,6. 02 and 4. 88,P〈0. 05）. The total review rate of the XN-9000（ WDF/WPC） was significantly lower than that of the XE-2100（ χ^2= 4. 33,P〈0. 05）.Similarly,the review rate of the XN-9000 for the blast/abnormal lymphocyte alarm（ 84/260,32%） was significantly lower than that of the XE-2100（ 107/260,41%）（ χ^2= 4. 38,P〈0. 05）. Conclusion Compared with the XE-2100,the XN-9000 WPC channel can improve the overall efficiency of the blast/abnormal lymphocyte alarm,reduce the false positive rate and the review rate,and then improve the efficiency of clinical laboratories.
出处
《临床检验杂志》
CAS
CSCD
2017年第2期101-104,共4页
Chinese Journal of Clinical Laboratory Science
关键词
血液分析仪
白细胞
前体白细胞通道
hematology analyzer
white blood cell
white precursor cell channel
