摘要
目的:探索人远端上游元件结合蛋白(FUBP1)在肾透明细胞癌(ccRCC)中的表达水平及其临床意义。方法:通过实时荧光定量PCR(real-time PCR)、蛋白免疫印迹实验(Western blot)和免疫组织化学检测FUBP1在ccRCC肿瘤组织、对应的瘤旁组织的表达情况。Western blot检测不同细胞系(786-O、Caki-1和HKC)之间FUPB1的表达情况。结果:在ccRCC肿瘤组织中,FUBP1的mRNA表达水平明显高于其对应的瘤旁组织(P<0.05),蛋白表达情况一致。且在ccRCC肿瘤细胞中FUBP1表达水平较高。FUBP1的mRNA表达水平增高,肿瘤分期较高、体积较大。结论:FUBP1可能在ccRCC肿瘤形成中发挥作用,FUBP1的高表达促进ccRCC肿瘤形成。
Objective：To explore the expression and significance of FUBP1 in clear cell renal cell carcinoma（ccRCC）.Method：FUBP1 expression was detected in ccRCC tissues and their corresponding adjacent normal renal tissues by real-time RT-PCR,Western blot analysis and immunohistochemistry.The expression of FUBP1 was studied in cell lines as well.The correlations between FUBP1 mRNA expression levels and clinicopathological factors were evaluated.Result：The levels of FUBP1 mRNA and protein expression were upregulated in human ccRCC tissues compared with adjacent noncancerous tissues（P〈0.05）.And the expression of FUBP1 was higher in cell lines 786-O and Caki-1.High levels of FUBP1 mRNA expression were associated with higher tumor stage and larger tumor size.Conclusion：FUBP1 may act as an oncogene in ccRCC.The upregulation of FUBP1 may promote tumorigenesis of ccRCC.
作者
巫胜攀
马鑫
李宏召
段珺耀
王瀚锋
唐露
彭程
张旭
WU Shengpan MA Xin LI Hongzhao DUAN Juanyao WANG Hanfeng TANG Lu PENG Cheng ZHANG Xu(Medical School of Chinese PLA, Beijing, 100853, China Department of Urology, Chinese PLA General Hospital, Beijing, 100853, China)
出处
《临床泌尿外科杂志》
2017年第3期192-195,共4页
Journal of Clinical Urology
