摘要
目的:通过研究类风湿关节炎(RA)患者血清IL-37和可溶性PD-1分子的表达水平,初步探讨其与RA的相关性以及临床意义。方法:收集RA患者及对照组人群的外周血,然后采用酶联免疫吸附法(ELISA)检测30例RA患者(RA标准评分≥6)和30名健康对照组血清中IL-37、s PD-1的表达水平,结合分析两组人群外周血IL-18、IL-6和IL-18BP三种细胞因子的表达,并通过Pearson相关分析其相关性。结果:经ELISA检测发现,RA组患者外周血中IL-37和s PD-1以及其他几种炎症相关细胞因子IL-18、IL-18BP、IL-6的表达水平均高于健康对照组(P<0.05);且IL-37与细胞因子IL-18、IL-18BP、IL-6均呈正相关,s PD-1仅与细胞因子IL-6呈正相关,IL-37、s PD-1均与患者病情程度评分呈正相关。结论:IL-37、s PD-1在RA患者中表达水平升高,且与其他炎性细胞因子及疾病严重程度具有相关性,两者在RA的进展中可能发挥着重要的调控作用,为今后RA治疗提供新的依据。
Objective： To investigate the relationship between IL-37 and soluble PD-1 in patients with rheumatoid arthritis（ RA）,and investigate the relationship between IL-37 and soluble PD-1. Methods： Peripheral blood was collected from RA patients and control group. The levels of IL-37 and s PD-1 in 30 RA patients（ RA standard score ≥ 6） and 30 healthy controls were measured by enzyme-linked immunosorbent assay（ ELISA）. The expression of IL-18,IL-6 and IL-18 BP in the peripheral blood of two groups was analyzed and the correlation was analyzed by Pearson correlation analysis. Results： The results of ELISA detection showed that the expression levels of IL-37 and s PD-1 in the peripheral blood of patients with RA and other inflammatory cytokines such as IL-18,IL-18 BP and IL-6 were higher than those of healthy controls（ P〈0. 05）. IL-37 was positively correlated with cytokines IL-18,IL-18 BP and IL-6; s PD-1 was positively correlated with cytokine IL-6. IL-37 and s PD-1 were positively correlated with the severity of patients. Conclusion： IL-37 and s PD-1 are elevated in RA patients and associated with other inflammatory cytokines and disease severity. Both of them may play an important regulatory role in the progress of RA,and provide a new basis for future RA therapy.
作者
陈栖栖
田娟
张晶
苏江
CHEN Xi-Xi TIAN Juan ZHANG Jing SU Jiang(Department of Rheumatology and Immunology, Sichuan Academy of Medical Sciences ＆ Sichuan Provincial People＇s Hospital, Chengdu 610072, China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期422-425,共4页
Chinese Journal of Immunology
基金
四川省卫计委科研课题资助(No.120071)
