摘要
目的:探讨禽流感病毒单克隆抗体的生物学特性。方法:制备H5N1禽流感病毒的单克隆抗体,鉴定其亚型、效价、血凝抑制活性以及与其他亚型流感病毒的交叉反应性。免疫印迹方法验证单抗与H5N1抗原的结合,人体正常组织芯片的免疫组织化学染色法观察单抗的组织学反应。结果:发现两株禽流感病毒H5N1的单抗(H5-32和H5-35)分别与人体的肾、胰腺组织存在阳性结合。结论:禽流感病毒H5N1与人体组织间可能存在某种关联,这或许可为禽流感病毒感染和致病研究提供参考。
Objective： To investigate the biological characteristics of monoclonal antibodies against avian influenza virus（ AIV）. Methods： Monoclonal antibodies（ m Abs） against AIV H5N1 were prepared and it’s characteristics were identified including subtype,titer,hemagglutination inhibition activity and cross-reactivity with other influenza viruses. Besides,Western blot and immunohistochemical staining methods were conducted to test the combination of antibodies and antigen（ H5N1） and human normal tissues. Results： Immunohistochemical analysis showed that 2 m Abs（ H5-32 and H5-35） cross-reacted with human tissues kidney and pancreas respectively. Conclusion： These data indicated that there have some association between the AIV H5N1 with human tissues,which may provide reference for the study on avian influenza virus infection and pathogenicity.
作者
李静丽
张海祥
冯杨萌
王光华
刘永年
胡军
LI Jing-Li ZHANG Hai-Xiang FENG Yang-Meng WANG Guang-Hua LIU Yong-Nian HU Jun.(Department of Pathophysiology , Medical College, Qinghai University ,Xining 810000, China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期398-400,406,共4页
Chinese Journal of Immunology
基金
"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(No.2014ZX10004002)
国家重点研发计划项目"畜禽重大疫病防控与高效安全养殖综合技术研发"重点专项(No.2016YFD0500700)资助
关键词
禽流感病毒
单克隆抗体
特性
鉴定
Avian influenza virus
Monoclonal antibody
Characteristic
Identification
