摘要
目的:探索抗IL-9抗体治疗小鼠肝癌恶性腹水(MA)的疗效及作用机制。方法:应用小鼠H22细胞系建立小鼠MA模型,造模24 h后将45只小鼠随机分成实验组、阴性对照组和空白对照组,每组15只。实验组给予腹腔注射抗IL-9抗体,阴性对照组给予注射同型Ig G抗体,空白对照组给予注射生理盐水,隔天1次,共注射5次。每次注射前均称量各组小鼠体重,并观察小鼠日常活动状态。末次用药结束24 h后每组随机处死5只小鼠,测量MA体积,收集MA上清,采用ELISA法检测MA中VEGF、MMP-2、IL-9、IFN-γ表达水平。其余小鼠观察其生存时间。结果:干预后实验组、阴性对照组和空白对照组小鼠的MA体积分别为(6.70±1.52)ml、(10.28±1.75)ml、(10.36±2.30)ml,与空白对照组和阴性对照组相比,实验组小鼠MA体积明显减少,差异有统计学意义(P<0.05)。实验组小鼠平均生存时间为(17.2±2.1)d,显著长于阴性对照组(14.5±1.2)d和空白对照组(14.8±1.4)d(P<0.05);与空白对照组和阴性对照组相比,实验组小鼠MA中VEGF水平显著降低,差异有统计学意义(P<0.05),与阴性对照组相比,实验组小鼠MA中IL-9水平显著降低,差异有统计学意义(P<0.05);但三组间MMP-2、IFN-γ水平比较差异无统计学意义(P>0.05)。结论:腹腔注射抗IL-9抗体可以有效地抑制H22细胞荷瘤小鼠MA的产生,延长其生存时间,该作用可能是通过抑制MA中VEGF、IL-9的表达而实现。
Objective： To explore the effects and mechanism of anti IL-9 antibody on malignant ascites（ MA） of hepatic carcinoma in mice. Methods： A mouse model of MA was established by mouse H22 cell line. 45 mice were divided randomly into experimental group,negative control group and blank control group at 24 hours after modeling,with 15 mice in each group. The experimental group was injected intraperitoneally with anti IL-9 antibody; the negative control group was injected with isotype Ig G antibody; the blank control group was injected with normal saline. The weight and behavior of the mice were measured before each injection. Five mice of each group was sacrificed at 24 hours after the last injection to measure the volume of MA. The level of VEGF,MMP-2,IL-9 and IFN-γ in MA were determined with ELISA assay; the survival time of rest mice were recorded and compared. Results： The mean volume of MA of experimental group,negative control group and blank control group were（ 6. 70 ±1. 52） ml,（ 10. 28 ± 1. 75） ml,（ 10. 36 ± 2. 30）ml,respectively,the MA volume of experimental group were lower as compared to negative control group and blank control group（ P 〈0. 05）. The mean survival time of experimental group was（ 17. 2±2. 1） d,which was significantly prolonged compared with the negative control group（ 14. 5±1. 2） d and the blank control group（ 14. 8±1. 4） d（ P〈0. 05）. The levels of VEGF of MA in experimental group was significantly lower compared to the negative control group and blank control group（ P〈0. 05）. The levels of IL-9 of MA in experimental group was significantly lower compared to the negative control group（ P〈0. 05）. The levels of MMP-2 and IFN-γ in experimental group had no significant difference compared with the negative control group and blank control group（ P〉0. 05）. Conclusion： Intraperitoneal injection anti IL-9 antibody on H22 ascites-bearing mice can effectively inhibit the generation of the MA. The mechanism may be related to the inhibition of the expression of the VEGF and IL-9.
作者
张沛玲
雷荣娥
覃沁怡
石城
姜海行
覃山羽
ZHANG Pei-Ling LEI Rong-E QIN Qin-Yi SHI Cheng JIANG Hai-Xing QIN Shan-Yu.(Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期388-391,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(No.31560257
31360221)资助
