摘要
目的:研究精氨酸单糖苷(Arginyl-fructose,AF)对环磷酰胺(CTX)诱导的免疫抑制小鼠免疫功能的影响。方法:ICR小鼠100只,随机分成5组,即正常对照组(N),免疫抑制模型组(M),免疫抑制AF低、中、高剂量组(M-L、M-M、MH)。试验组连续给药28 d。免疫抑制组,每周前3天,腹腔注射80 mg/kg环磷酰胺,形成免疫抑制。末次给药12 h解剖后,测定免疫器官指数,AF对脾淋巴细胞转化及增殖、巨噬细胞吞噬功能、特异性Ig G抗体、血清中TNF-α、IL-2含量的影响。并通过实时荧光定量PCR检测TNF-α、IL-2基因表达的差异。结果:AF能够显著提高小鼠的胸腺指数和脾脏指数,促进脾淋巴细胞的自然转化与增殖,增强巨噬细胞吞噬功能,显著提高小鼠血清中特异性Ig G抗体含量,可显著提高小鼠血清中TNF-α、IL-2含量,实时荧光定量PCR检测结果显示,TNF-α、IL-2 mRNA能够良好表达。结论:AF能拮抗CTX免疫功能低下小鼠的免疫抑制作用。
Objective： To study the effect of AF on mice induced by cyclophosphamide. Methods： 100 ICR mice were divided into 5 groups randomly,the normal contral group（ N）,immunosuppressive model group（ M）,AF low-dose group（ M-L,20 mg / kg）,AF middle-dose group（ M-M,40 mg / kg）,AF high-dose group（ M-H,80 mg / kg）. Drugs were chronically administered to mice for 28 days,dosage of administration was 10 ml / kg of the mice’ s weight. The groups of M,M-L,M-M,M-H received an injection of cyclophosphamide（ CTX,80 mg / kg） three times a week to cause immunosuppressive. Organ idex,splenic lymphocyte transformation,phagocytic function,Ig G,IL-2,TNF-α were conducted 12 hours after the last administration in mice of each group. Results： AF could improve the thymus index and spleen index in mice significantly,promoting the natural transformation and proliferation of splenic lymph-ocytes,adding phagocytic function,AF could improve the content of Ig G,IL-2,TNF-α in serum significantly. The result of Real-time fluorescence quantitative PCR show that TNF-α mRNA,IL-2 could be well expressed. Conclusion： AF can inhibit hypoimmunologic mice induced by CTX.
作者
宋明铭
王佳奇
黄宝亮
高铭彤
李婧毓
丁传波
郑毅男
刘文丛
徐晓华
SONG Ming-Ming WANG Jia-Qi HUANG Bao-Liang GAO Ming- Tong LI Jing- Yu DING Chuan-Bo ZHENG Yi- Nan LIU Wen-Cong XU Xiao-Hua(Jilin Agricultural University, Changchun 130118, China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期347-351,共5页
Chinese Journal of Immunology
基金
吉林省自然科学基金(20160101017JC)
吉林大学生创业资金项目(20160521011HJ)
延边州科技发展计划项目(2015JH01)资助
