摘要
为筛选出具有抑菌活性且次级代谢产物丰富的红树林内生真菌,以水产养殖中分离得到的2株病原细菌(Vibrio parahaemolyticus B1和Acinetobacter johnsonii B2)对2014年从珠海淇澳-担杆岛省级自然保护区采集得到的616株红树林内生真菌进行代谢产物抗菌活性和丰富度筛选。利用共培养技术,寻找在共培养后菌落形态特征出现明显变化或形成抑菌圈的内生真菌。通过对纯培养和共培养真菌的粗提物进行抑菌活性检测和HPLC指纹图谱分析,筛选出在共培养条件下可诱导产生新代谢产物且抑菌效果显著提高的菌株,为发现抗菌化合物提供新来源。
In order to screen out mangrove endophytic fungal strains with antibacterial activity and abundant secondary metabolites,two strains of aquatic pathogenic bacteria named as Vibrio parahaemolyticus B1 and Acinetobacter johnsonii B2 were co-cultured with 616 strains of mangrove endophytic fungi,which were collected from Guangdong Zhuahai Qiao-Dangan island nature reserve in 2014,respectively. And then some of the endophytic fungi with obvious changes in the morphology of the colonies or produce inhibition zones after co-culture were investigated. The crude extracts of pure cultures and co-cultures were analyzed by HPLC fingerprints to find out some fungal strains that could induce new secondary metabolites and increase antibacterial effect in co-culture conditions. In addition,their antibacterial activity against V. parahaemolyticus B1 and A. johnsonii B2 were evaluated. It is a new strategy for these strains to produce novel antibacterial compounds.
作者
张柳红
李嫣然
李云龙
宁嘉盈
周耀航
熊智瑶
李佳欣
王玉如
黄志坚
刘岚
ZHANG Liuhong LI Yanran LI Yunlong NING flaying ZHOU Yaohang XIONG Zhiyao LI Jiaxin WANG Yuru HUANG Zho＇ian LIU Lan(School of Marine Sciences, Sun Yat-sen University, Guangzhou 510006, China)
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第2期93-101,共9页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金(21272286)
海洋公益性行业科研专项经费(201305017)
海洋生物天然产物化合物库基金(GD2012-D02-001)
中山大学2015年大学生创新训练和创业训练计划项目(教务[2015]105号)
