摘要
目的探讨P38丝裂原活化蛋白激酶(P38MAPK)及双特异性磷酸酶9(DUSP9)与早发型重度子痫前期发生发展的关系,揭示其与细胞外信号调节蛋白激酶(ERK)1/2信号通路相互作用机制,为早发型重度子痫前期的发病机理提供理论依据。方法收集122例重度子痫前期患者为研究组(其中早发型62例,晚发型60例),随机抽取同期65例正常妊娠者为对照组。采用免疫组化方法检测胎盘组织中P38MAPK、DUSP9的表达水平。结果各组胎盘组织的合体滋养细胞、蜕膜细胞、血管内皮细胞中均可检测到P38MAPK、DUSP9的表达。早发型重度子痫前期组P38MAPK表达显著高于晚发型重度子痫前期组及对照组(0.112±0.025,0.072±0.002,0.048±0.008,P<0.01);早发型重度子痫前期组DUSP9表达水平显著低于晚发型重度前期组及对照组(0.041±0.004,0.078±0.002,0.126±0.008,P<0.01);各组胎盘组织中P38MAPK与DUSP9的表达水平呈负相关(r=-0.53,P<0.05)。结论早发型重度子痫前期组胎盘中P38MAPK高表达及DUSP9低表达与其疾病的发生发展密切相关。提示P38MAPK、DUSP9/ERK1/2信号通路可能相互作用,并共同在早发型重度子痫前期发生发展中起重要作用。
Objective Study the relationship between P38MAPK, DUSP9 and early onset severe preeclampsia, re- veal the two factors and extracellular signal regulated protein kinase （ERK） , and reveal the foundation of the disease, and provide a theoretical basis of pathogeny. Methods The study groups include sixty - two early onset severe preeclampsia ones, and sixty later onset severe preeclampsia ones, and the control group include sixty -five normal deliveries. We detect the different expression levels of P38MAPK and DUSP9 in placenta with the methods of Immunohistochemistry. Results We can test the expression levels of P38MAPK and DUSP9 in the syneytiotrophoblast, vascular endothelial cells and decidual cells of the placenta. The expression level of P38MAPK is significantly higher in early onset severe preeclampsia ones than the levels in later onset severe preeclampsia ones and normal ones （0. 112 -＋0. 025, 0. 072 ＋0. 002, 0. 048 -＋0. 008, P 〈 0. 01 ）. The expression level of DUSP9 is significantly lower in early onset severe preeelampsia ones than the levels in later onset severe preeelampsia ones and normal ones （0. 041 _＋0. 004, 0. 078 -＋0. 002, 0. 126 ＋0. 008, P 〈0. 01 ）. The expres- sion levels of P38MAPK and DUSP9 are negative correlation （ r = - 0. 53, P 〈 0. 05 ）. Conclusions It is closely related to the pathogenesis and development of diseases with the high expression of P38MAPK and the low expression of DUSP9 in early onset severe preeclampsia placentas. Signaling pathways of P38MAPK and DUSP9 may interact with each other and play an important role in the development of early onset severe preeclampsia.
出处
《现代医院》
2017年第2期224-226,共3页
Modern Hospitals
基金
广东省科技计划项目(编号:2014A020212548)
