摘要
以来自黑龙江省、吉林省、辽宁省、北京、山东省、江苏省、湖北省和广东省8个省份的36株黄瓜霜霉菌为试材,采用SSR分子标记的方法,研究了黄瓜霜霉病菌的遗传多样性。结果表明:中国黄瓜霜霉菌遗传分化较大,群体遗传多样性比较丰富。利用11对SSR引物共扩增出170条谱带,其中多态性条带160条,占总带数的94.1%。从11对引物中筛选出了多态性好、条带清晰的引物D13。通过NTSYS软件构建了黄瓜霜霉菌的亲缘关系树状图,聚类分析结果表明,菌株间的相似系数在0.43~1.00,说明SSR遗传多样性与菌株的地理来源存在一定的相关性。
Thirty-six isolates of cucumber downy mildew from Heilongjiang,Jilin,Liaoning,Beijing,Shandong,Jiangsu,Hubei,and Guangdong Province were amplified.Using SSR molecular marker method studied the SSR genetic diversity analysis of Pseudoperonospora cubensis.The results showed that China had bigger genetic differentiation,and abundant genetic diversity of Pseudoperonospora cubensis.In total of 170 bands were obtained,including 160 polymorphic bands accounting for 94.1%.D13 were screened as the best polymorphic primer from 11 primers.The NTSYS software was used to build the relationship dendrogram of P.cubensis,cluster analysis showed that the coefficient of similarity was from 0.43 to 1.00.There were certain correlations between SSR genetic diversity and geographical origin.
出处
《北方园艺》
CAS
北大核心
2017年第6期130-134,共5页
Northern Horticulture
基金
国家自然科学基金资助项目(31171792)
黑龙江省自然科学基金重点资助项目(ZD2016003)
关键词
黄瓜霜霉菌
SSR
遗传多样性
Pseudoperonospora cubensis
SSR
genetic diversity
