摘要
目的：研究湖南产乌药化学成分。方法：分别采用气相色谱-质谱（GC-MS）联用法和液相色谱-质谱（UPLC-ESI-MS/MS）对乌药中挥发油和甲醇提取物的化学成分进行分析。在GC-MS中,以HP-5MS毛细管柱（30 m×0.25 mm,0.25μm）为色谱柱,采用程序升温法,EI离子源,扫描质量范围为m/z 35～400 amu;在UPLC-ESI-MS/MS中,以Acquity UPLC BH C18（50 mm×2.1 mm,1.7μm）为色谱柱,采用0.1%甲酸水溶液-乙腈为流动相,梯度洗脱,ESI源,扫描质量范围为m/z 100～1 200。结果：利用GC-MS法共鉴定了乌药中68个挥发性成分,主要为单萜类物质。利用UPLC-ESI-MS/MS法在甲醇提取物中共鉴定出42个化合物,其中24个呋喃倍半萜及其内酯、10个生物碱、6个黄烷醇多酚及其缩合物、1个有机酸和1个甾醇类化合物。结论：系统研究了湖南产乌药中的化学成分,为湖南产乌药的药效物质基础及质量控制研究提供一定的理论依据。
Objective： To study the chemical composition of Lindera aggregate produced in Hunan. Methods： Volatile oil of Lindera aggregata and chemical composition of methanol extract were analyzed by GC-MS and UPLC-ESI-MS / MS. The GC-MS condition was as follows,HP-5MS capillary column（ 30 m × 0. 25 mm,0. 25 μm） for column chromatography,temperature-programmed and EI were used,the scanning range of quality was m / z 35 ~ 400 amu; the UPLC-ESI-MS / MS conditions were as follows,Acquity UPLC BHC18（ 50 mm ×2. 1 mm,1. 7 μm） with mobile phase of 0. 1% formic acid aqueous solution and acetonitrile under gradient elution,with the EI source. The scanning range was m / z 100 ~ 1 200. Results： 68 volatile compounds were identified in Lindera aggregata,mainly monoterpene substance,42 compounds were identified,24 furan sesquiterpene and lactone,ten alkaloids,six flavonol polyphenol and condensates,an organic acid and a sterol classes of compounds in the methanol extract of Lindera aggregata. Conclusion： This study make a comprehensive understanding of compounds of Lindera aggregata which produced in Hunan,and provides a theoretical basis for pharmacodynamic substance foundation and the quality control of Lindera aggregata.
作者
邓桂明
向彪
肖小芹
葛金文
陈镇
杨立平
魏凤
DENG Gui-ming XIANG Biao XIAO Xiao-qin GE Jin-wen CHEN Zhen YANG Li-ping WEI Feng(The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China The College of Integrated Traditional Chinese and Western Medicine of Hunan University of Chinese Medicine, Changsha 410208, China Hunan Cancer Hospital, Changsha 410013, China)
出处
《中药材》
CAS
CSCD
北大核心
2016年第10期2229-2236,共8页
Journal of Chinese Medicinal Materials
基金
中国博士后基金(2016T90752
2014M550420)
湖南省教育厅重点项目(14A107)
湖南省教育厅项目(15C1041)
湖南省中医药科研计划项目(201429
2013135)
湖南省研究生科研创新项目(2014CX02)
