摘要
目的 了解淮安市某中学一起引起聚集性感染的流感病毒HA1基因特征.方法 采用实时荧光RT-PCR方法进行病原体的快速检测,对分离的毒株进行HA1基因扩增、测序及构建系统发生树并进行HA1基因特征分析.结果 从流感样病例中检测出11份甲型H1N1流感病毒阳性,与疫苗株A/California/07/2009核苷酸及氨基酸同源性分别为97.7％-98.1％及96.6％-97.4％.引起本次疫情的甲型H1N1流感病毒株与2014年分离的毒株聚集在一起.序列分析提示淮安分离株受体结合位点及糖基化位点的氨基酸序列均未发生变异,在抗原决定簇Ca1区均发现变异,为S220T.结论 引起本次聚集性疫情的病原体为甲型H1N1流感病毒,尽管淮安株在抗原决定簇Ca1区存在氨基酸突变,但其抗原性未发生改变.
Objective To understand the viral etiology of a clustered case of human infection outbreak in the middle school of Huai＇an city.Methods Nasopharyngeal swab samples from patients were collected and rapidly detected by Real-time RT-PCR and the target virus isolated in cells.Furthermore,HA1 segments of target virus were amplified by RT-PCR and sequenced.The genetic and phylogenetic analysis based on HA1 genes was computed.Results Influenza A （H1N1）pdm09 viral nucleic acid in 11 nasopharyngeal swab samples from patients in the outbreak were positive.Compared to the vaccine strains A/ California/07/2009,the Huai＇an isolates,nucleotide identity was 97.7%-98.1%,and amino acid identity was 96.6%-97.4%.Phylogcnetic analysis of HA1 segment scqucnccs indicatcd that the Huai＇an strains from the outbreak were related closely to the viruses isolated in the year of 2014.Sequence analysis indicated that the Huai＇an isolates had no amino acid substitution in the receptor binding sites and glycosylation sites,while in the Ca1 of antigenic determinant of HA1 the Huai＇an isolates had an amino acid substitution of S for T at 220.Conclusions The pathogen of the clustered case of human infection was Influenza A（H1N1） pdm09 virus.Though the Huai＇an isolates had one animo acid substitution in the Ca1 of antigenic determinant,the antigenicity characteristic remained unchanged.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2017年第1期42-46,共5页
Chinese Journal of Experimental and Clinical Virology
基金
国家传染病重大科技专项（2013ZX10004-101-006）
淮安市预防医学会科研基金（hayt201516）
淮安市科技局科研项目（HAS2015019-3）
