摘要
合成了CS-PB-GR复合物,并将其修饰在玻碳电极表面,通过共价作用结合Pt/Au纳米合金,进一步固载甲胎蛋白（AFP抗体）,采用HRP进行封闭,构建高灵敏电化学免疫传感器。采用示差脉冲伏安法（DPV）和循环伏安法（CV）对电极修饰过程及响应性能进行研究。在最佳实验条件下,电流响应值与AFP浓度在0.1~200 ng/m L的范围内呈线性关系,检出限为0.03 ng/m L,具有重现性、稳定性好的特点,可实现对免疫分子的高灵敏检测。
The synthesis of chitosan-Prussian blue-graphene（CS-PB-GR） was conducted firstly,followed by the colvantly linked Pt/Au nanomaterials.Then,anti-AFP could be absorbed to the electrode surface by the reaction between Au and amino.After HRP blocked,the electrochemical immunosensor was prepared successfully,which were demonstrated by differential pulse voltammetry（DPV） and cyclic voltammetry（CV）.The effects of experimental parameters and the performance of the biosensor were also been investigated.under the optimal experiment conditions,the prepared immunosensor displayed a liner relationship between 0.1~200 ng/m L with a detection limit of 0.03 ng/m L.The proposed immunosensor could be used for the detection of immune molecular with high sensitivity,good stability and selectivity.
出处
《化学传感器》
CAS
2016年第4期49-53,共5页
Chemical Sensors
基金
国家自然科学基金(81401757)
发育与再生四川省重点实验室项目(SYS16-004)
