摘要
为实现低温生物甲烷高效转化与发酵系统稳定运行,应探索提高低温沼气发酵细菌代谢能力途径与冷适机制。研究以发酵温度(10、15、18、22、25°C和30°C)为驱动因子,人工培养基定向富集自然低温生境混合菌群,考察沼气发酵细菌低温生物甲烷转化效能,并耦合454高通量测序和PCR-DGGE解析低温混合菌群落结构。结果表明,低温生境混合细菌最优势细菌类群为Clostridium XI sp.,占细菌总数的40.9%,确定分类地位的产甲烷菌有Methanosaeta sp.和Methanobacterium sp.。低温沼气发酵细菌在18°C富集培养的沼气产量最高,可达220 ml L^(-1)reactor d^(-1),甲烷含量为56.8%。
Improving the metabolic activity of psychrophilic biogas-producing bacteria is crucial to realize efficient biomethane conversion and stable operation of anaerobic digester under low temperature. The study adopts fermentation temperature（10、15、18、22、25 °C and 30 °C） as limited factor to investigate biogas conversion of psychrophilic bacteria cultivated from natural habitat using oriented enrichment of artificial medium. Furthermore, 454 pyrosequencing technology and PCR-DGGE were employed to analyze microbial community diversity. The results show that the dominant psychrophilic bacteria is Clostridium XI sp. and accounts for 40.9%. The Methanosaeta sp. and Methanobacterium sp. were detected from psychrophilic bacteria. The optimum biogas production was 220 ml L～（-3） reactor d～（-1） and 56.8% of methane content operated under 18 °C.
作者
赵光
郭星
冀丽爽
邓建民
马放
ZHAO Guang GUO Xing JI Li-shuang DENG Jian-min MA Fang(Chemical and Environrmtal Engineering college, Liaoning University of Technology, Jinzhou 121001, China Hailin Farm, Hailin 150000, China School of Municipal Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China)
出处
《辽宁工业大学学报（自然科学版）》
2017年第1期48-51,61,共5页
Journal of Liaoning University of Technology(Natural Science Edition)
基金
国家科技支撑计划专题项目(2012BAD14B06-04)
辽宁省教育厅项目(L2014236)
辽宁工业大学教师科研启动基金(X201310)
