摘要
为改进双波长分光光度计法测定烟草淀粉组分的前处理,参照其他作物淀粉组分的测定方法,结合烟草淀粉的特殊来源,借助索氏提取器将脂肪、色素、糖类等对淀粉测定有影响的物质除去,然后对样品中淀粉组分含量进行测定。结果表明,前处理改进后所得样品的重复性试验相对偏差小,待测样品溶液稳定性良好;加标样直链淀粉的平均回收率为103.71%。改进后的前处理方法操作便捷,适用于烟草制品批量检测。
To improve the pretreatment of determination of the tobacco starch components by dual wavelength spectrophotometry,we referenced the measurement methods of other crops and considered its specific source,and used Soxhlet extraction System to remove the fat,pigmentum,sugars and other substances that affect the determination of starch components,then determined the starch component content in the samples. The results showed that the relative standard deviation of the repeated test of the sample from the improved pretreatment is small and the stability of the sample solution is good. The recovery rate of added mylose was 103. 71%. The improved pretreatment program is simple,and easy to operate,and the new method can be applied for analyzing plentiful samples due to its accuracy and rapidness.
作者
杨胜男
牛德新
张洪映
连文力
张权
张晓阳
杨新士
崔红
YANG Shengnan NIU Dexin ZHANG Hongying LIAN Wenli ZHANG Quan ZHANG Xiaoyang YANG Xinshi CUI Hong(College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, China Qingzhou Cigarette Factory, China Tobacco Shandong Industial Co. , Ltd. , Qingzhou 262500, China Shicheng County Tobacco Company of Jiangxi Province, Shicheng 341000, China)
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2017年第1期8-12,共5页
Journal of Henan Agricultural University
基金
中国烟草总公司特色优质烟叶开发重大专项(110200902045TS-01)
关键词
烟草
淀粉组分
双波长分光光度法
前处理改进
tobacco
starch components
dual wavelength spectrophotometry
improved pretreatment
