摘要
胞外囊泡(EVs)是由多种类型细胞释放的微小囊泡。目前研究认为EVs是细胞间信息传递的重要介质,可通过传递蛋白质、信使RNA(m RNA)、微小RNA(micro RNA,mi RNA)等改变靶细胞的基因表达、蛋白质合成等生物学功能,在组织修复、生物标记、疾病诊断等方面均发挥着越来越重要的作用。目前,分离提取EVs的主要方法有:差速离心法、超滤法和免疫亲和层析法,各有优缺点。在实际工作中,研究者常将多种分离提取方法联合应用以提高产物纯度,稳定EVs生物活性,减少提取时间。近年发现,在卵泡液和精液中存在EVs,其生物学意义及潜在的临床价值有待于进一步研究。本文介绍EVs提取方法的研究进展,为EVs的研究提供参考。
Extracellular vesicles （EVs） are small vesicles released from various cells, which play an important role in cell to cell communication via transmitting proteins and genetic materials including DNA, mRNA and microRNA （miRNA） from original cell to target cell. EVs participate in many physiological and pathophysiological processes such as tissue regeneration, suggesting that EVs may be used as a biological marker for disease diagnosis. Currently, the frequently-used methods of EVs isolation were differential centrifugation, ultrafiltration and immunoaffinity. Several methods are always combined to increase the purity of isolated EVs, to stabilize their activities, and to reduce isolation time. Recent research reported that EVs were also isolated from semen and follicular fluid. It is necessay to explore the biological and clinical significance of EVs in semen and follicular fluid. The research progress on the isolation methods of EVs, and the advantage and disadvantage of each method were summarized in the present review as a reference for our EVs study.
出处
《国际生殖健康／计划生育杂志》
CAS
2017年第2期125-129,共5页
Journal of International Reproductive Health/Family Planning
基金
南京军区医学科技创新项目(11Z010
14ZX06)
关键词
脂质体
细胞分离
离心法
超滤
色谱法
亲和
卵泡液
精液
Liposomes
Cell separation
Centrifugation
Ultrafiltration
Chromatography, affinity
Follicular fluid
Semen
