摘要
目的:研究缝隙连接蛋白36(CX36)在癫痫模型中的表达变化及其阻滞剂奎宁在癫痫发生、发展中的作用。方法:应用锂-匹罗卡品法制造幼鼠癫痫模型,并在干预组用奎宁进行特异性阻滞。应用尼氏染色法观察1、3、24 h模型组与奎宁干预组海马神经元的受损情况;采用免疫组织化学显色及免疫印迹检测各组海马中CX36的动态表达。结果:尼氏染色可见癫痫发作1 h即可出现神经元的缺失、损伤,至24 h达到巅峰。奎宁干预组较模型组神经元受损情况明显减轻。模型组各时间点CX36表达增多,致癫痫3 h达到高峰,之后下降。奎宁干预组各时间点CX36表达较实验组降低。结论:CX36可能参与幼鼠癫痫的发生发展过程、可能参与了神经元损伤过程。
Objective： To study the expression of connexin 36 （CX36） in epilepsy model and to investigate quinine＇s role in epilepsy. Methods： Lithium-pilocarpine method was used to produce the epilepsy model of young rats, and quinine was used in the intervention group. Neuron damage in the model group and quinine treated group after seizure for 1 h, 3 h, 24 h by Nissl＇s staining was observed, and the dynamic expression of CX36 in the hippoeampus of these groups was measured by immunohistochemistry and Western blotting. Results： Nissl＇s staining showed the neuron damage began to appear in the hippocampus in the model group 1 h after seizure, and reached the peak 24 h after seizure. The damage of quinine treated group was significantly more serious than the model group. The expressions of CX36 increased in all the subgroups, and reached the peak 3 h after seizure. In all the subgroups of quinine treated group, the expression of CX36 was less than those in the model group. Conclusion： CX36 may be involved in the occurrence and development of epilepsy, and may play an important role in the process of neuron damage.
作者
杨加尉
刘雪雁
Yang Jiawei Liu Xueyan(Department o f Pediatrics, Shengjing Hospital , China Medical University, Shenyang 110004, China)
出处
《解剖学杂志》
CAS
CSCD
北大核心
2017年第1期36-39,51,共5页
Chinese Journal of Anatomy
基金
辽宁省自然科学基金(2013021028)
辽宁省"百千万人才工程"资助项目(2013921026)
