摘要
植物中的漆酶(LAC)作为蓝铜氧化酶家族的一个分支,可以聚合木质素单体形成木质素。拟南芥、番茄中miR397b可以调控LAC蛋白家族的表达,但验证棉花中miR397b生化功能的相关报道目前尚无。选取开花后25 d的陆地棉纤维材料,通过RLM-5'RACE试验,验证了对GhLAC4 mRNA的切割发生在miR397b和GhLAC4的互补区域,即证明了GhLAC4是miR397b的靶基因。本研究预示着GhmiR397b可能参与棉花纤维中木质素的形成过程,为研究GhLAC4在棉花纤维发育过程中的调控作用提供了基础。
It is reported that plant laecase enzymes （ LAC ）, a member of the blue copper oxidase family, canpolymerize lignin monomers into lignin, miR397b plays a role in the expression of LAC protein family inArabidopsis and Lycopersicon. However, there is no report about biochemical identification of miR397b function. Inthis study, RLM-5＇ RACE experiment was carried out on the cotton fiber sampled at 25 days post-anthesis invitro,and it was validated that cleavage of GhLAC4 transcripts was mediated by miR397b via a complementaryregion between them, and GhLAC4 was the targeted gene of GhmiR397b. These results suggested that GhmiR397bmight be involved in the lignin formation in cotton fiber, and provided an evidence for studies on the regulationrole of GhmiR397b during cotton fiber development.
作者
丁妍
王燕美
刘进元
DING Yan WANG Yan-mei LIU Jin-yuan(Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China)
出处
《上海农业学报》
CSCD
2017年第1期10-14,共5页
Acta Agriculturae Shanghai
基金
国家重点基础研究发展计划(2010CB126003)
国家转基因动植物研究项目(2011ZX08005-003
2011ZX08009-003)
