摘要
[目的]考察东太湖地区湿地水质与浮游细菌群落结构之间的关联,探索对湿地水质具有指示作用的微生物。[方法]应用高通量测序研究浮游细菌群落结构;通过相关性分析考察了浮游细菌群落多样性与水质的关联,并运用LEf Se分析定位不同水质水样的标志微生物。[结果]东太湖湿地浮游细菌主要分布于放线菌门(相对丰度34.78%)、变形菌门(26.30%)及蓝细菌门(19.28%)等7个门;水质指标综合得分与浮游细菌群落香农-威纳指数和辛普森指数分别显著相关(r=-0.597,p<0.05;r=0.694,p<0.01);蓝细菌门与伯克氏菌科的相对丰度在不同水质水样中差异最为显著(LDA得分>4.0)。[结论]东太湖地区,较高的浮游细菌多样性表征较好的湿地水质;蓝细菌相对丰度较高(27.80%)指示湿地水质较差,而伯克氏菌相对丰度较高(5.48%)指示湿地水质相对较好。
[ Objective] This study investigated the correlations between the community structure of planktonic bacteria and water quality in the wetlands around eastern Lake Tai ; several taxonomic unitswith the potential function as the bio -indicators of water quality were located. [ Methods] The community structure of planktonic bacteria in these wetlands were investigated through high -throughput sequencing. The correlations between diversity index of planktonic bacteria and water quality index were tested through correlation analysis. Biomarkers of each cluster of samples with different water quality were located using LEfSe analysis. [ Results] Planktonic bacteria in the wetlands around eastern Lake Tai mainly distribute in 7 phyla including Actinobacteria （ relative abundance = 34.78% ） , Proteobacteria （ 26.30% ）, and Cyanobacteria （ 26.30% ）. The composite score of water quality index is significantly correlated to the Shannon - winner index and Simpson index of planktonic bacteria （r = -0. 597 ,p 〈 0.05 and r=0. 694 ,p 〈 0.01, respectively）. Cyanobaeteria and Burkholderiaceae were considered as biomarkers of samples with different water quality （ LDA score 〉 4.0 ）. [ Conclusion] Higher diversity index of planktonic bacteria indicates better water quality in the wetlands around eastern Lake Tai;high relative abundance of Cyanobacteria （27.80%） indicates water quality inferiority, high relative abundance of Burkholderiaceae （5.48%）indicates better water quality.
出处
《生物技术》
CAS
北大核心
2017年第1期85-91,共7页
Biotechnology
基金
苏州市科技计划项目("基于微生物多样性的苏州湿地健康评价研究"
No.SS201616)
关键词
东太湖
湿地
浮游细菌
水质
生物指示物
蓝细菌
伯克氏菌
eastern Lake Tai, wetland, planktonic bacteria, water quality, bio - indicater, Cyanobacteria, Burkholderiaceae
