摘要
目的 2014年10月浙江省金华市荣光小学发生疑似病毒性脑膜炎暴发疫情,为及时查明病因,分析病原的分子特征并进行病原溯源。方法开展流行病学调查,采集患者脑脊液和咽拭子样本6份,检测人类肠道病毒(HEV)核酸和分离病毒,对HEV分离株VP1基因测序,进行同源性与进化分析。结果在全校328名学生中,发病7例,罹患率2.13%。患者年龄7~8岁;临床症状为发热、头痛、呕吐等;从5例患者6份样本中均检测到HEV核酸,其中3份脑脊液中分离到3株肠道病毒埃可30型(echovirus 30,E30);新分离株与E30原型株之间VP1区核苷酸和氨基酸同源性分别为81.8%和91.8%~92.1%;进化分析显示:新分离株位于E30 G基因亚型分支上,与2013年浙江省CHN/ZJ/RA-72/13株亲缘关系最近。结论引起2014年10月浙江省金华市荣光小学聚集性病毒性脑膜炎暴发疫情的病原为E30 G基因亚型病毒。
Objective To identify and trace the pathogen of a suspected viral meningitis outbreak in a primary school in Jinhua city of Zhejiang province in 2014. Methods Epidemiological investigation was conducted and 3 specimens of cerebrospinal fluids （CSF） and 3 of throat swab were sampled from suspected patients for isolation and detection of human enterovims （HEV） nucleic acid. Then VP1 genes of the isolated virus were sequenced for the construction of phylogenetic tree and homologous analysis. Results Totally 7 cases aged 7 to 8 years were identified among 328 pupils in the school, with a morbidity of 2. 13 %. All the cases had symptoms of headache, fever, and vomiting. Six specimens from 5 patients were positive for HEV nucleotide and 3 strains of echovirus 30 （E30） were isolated from CSF speci- mens. The homology of nucleotide sequences of the VP1 gene was 81.8% and that of amino acid sequences ranged front 91.8% to 92. 1% between the 3 isolated strains and the prototype strain Bastianni of E30 ,respectively. The phylogenetic tree of VP1 gene showed that the E30 strains isolated belong to the E30 G subtype and are of close homology to CHN/ ZJ/RA-72/13 strain isolated in Zhejiang province in 2013. Conclusion The viral meningitis outbreak among pupils in Jinhua city of Zhejiang province in 2014 was caused by echovirus 30 G genotype.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2017年第2期298-301,共4页
Chinese Journal of Public Health
基金
浙江省科技厅项目(2012C33063)
