摘要
目的：探讨 IL-33及 IL-33 mRNA 在强直性脊柱炎患者中的表达水平，分析其临床意义，从而为疾病的治疗及预后提供参考。方法选择强直性脊柱炎患者32例作为研究组，同时选择健康人30例作为对照组，采用实时定量 PCR 及酶联免疫吸附法分别检测两组研究对象外周血中 IL-33 mRNA 及血浆中 IL-33的水平。结果与对照组[（1．23±0．58）]相比，研究组外周血 IL-33 mRNA[（1．74±0．75）]的表达水平较高，差异有统计学意义（t ＝2．981，P ＝0．004）。研究组血浆 IL-33水平[（227．30±45．67）pg／mL]高于对照组[（114．70±39．58）pg／mL]，差异有统计学意义（t ＝10．344，P ＜0．01）。与对照组相比，研究组红细胞沉降率、CRP、血小板计数明显较高[（47．80±4．73）mm／h、（42．60±3．38）mg／L、（329．60±48．39）×109／L]（t ＝42.542、54．722、14．040，均 P ＜0．01）。血浆 IL-33水平、外周血 IL-33 mRNA 的表达水平与疾病活动度指数（BASDAI）（r ＝0．472、0．457，均 P ＜0．05）、CRP（r ＝0．713、0．687，均 P ＜0．05）呈正相关。结论强直性脊柱炎患者血浆 IL-33及外周血 IL-33 mRNA 水平显著升高，IL-33及 IL-33 mRNA 在强直性脊椎炎的发生、发展中可能发挥重要的作用。
Objective To explore expression of IL -33 and IL -33 mRNA in peripheral blood of patients with ankylosing spondylitis,and analyze the clinical significance,so as to provide scientific reference for the treatment and prognosis of the disease.Methods From February 201 4 to May 201 6,32 patients with ankylosing spondylitis were included as study group,30 healthy persons were selected as control group.Real -time quantitative PCR and enzyme -linked immunosorbent assay were used to detect IL -33 mRNA level in peripheral blood and IL -33 level in plasma of subjects.Results Compared with the control group （1 .23 ±0.58）,IL -33 mRNA in peripheral blood of patients in the study group was higher（1 .74 ±0.75）,and the difference was statistically significant （t =2.981 , P =0.004）.ELISA results showed that IL -33 level in plasma of the study group was higher （227.30 ±45.67）pg/mL than the the control group （1 1 4.70 ±39.58）pg/mL,the difference was statistically significant （t =1 0.344,P 〈0.01 ）.Compared with the control group,the ESR,CRP and PLT of the study group were significantly higher[（47.80 ± 4.73）mm/h,（42.60 ±3.38）mg/L,（329.60 ±48.39）×1 09 /L]（t =42.542,54.722,1 4.040,all P 〈0.01 ）.IL -33 in plasma and IL -33 mRNA in peripheral blood of ankylosing spondylitis patients were positively correlated with BASDAI（r =0.472,0.457,all P 〈0.05）,CRP（r =0.71 3,0.687,all P 〈0.05）.Conclusion IL -33 and IL -33 mRNA in patients with ankylosing spondylitis significantly increase,indicates that they should play important role in the occurrence and development of ankylosing spondylitis.
作者
龚韶华
石晓兵
包朝鲁
Gong Shaohua Shi Xiaobing Bao Chaolu(Department of Orthopaedics ,Baoshan District Hospital of Integrated Chinese and Western Medicine ,Shanghai 201900, China)
出处
《中国基层医药》
CAS
2017年第1期45-48,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
上海市进一步加快中医药事业发展三年行动计划建设项目（ZY3-FWMS-2-1001）
