摘要
目的检测基质金属蛋白酶（MMP-9）在结直肠癌中的表达情况,探讨其与肠癌浸润和转移的相关性。方法 2007年1月~2010年12月期间在南京医科大学第二附属医院和盐城市第三人民医院就诊的行手术治疗的99例结直肠癌患者作为研究对象。应用组织芯片技术结合免疫组化SP法检测结肠癌组织和自身正常肠黏膜组织中MMP-9的表达情况,分析肠癌的不同病理特征和组织中MMP-9表达之间的关系。结果 MMP-9在结直肠癌组织中的表达明显高于正常肠黏膜（P〈0.001）。在浸润深度深、有淋巴结转移、分期晚的患者中,癌组织中MMP-9的表达明显增高（P〈0.05）。癌组织中的表达与患者生存相关,癌组织MMP-9表达高的患者死亡比例高（P〈0.05）。结论 MMP-9在结直肠癌组织中的表达和肿瘤浸润深度、淋巴结转移正相关。MMP-9可作为结直肠癌进展的指标,用于判断预后。
Objective To investigate the expressions of matrix metalloproteinases（MMP-9） in colorectal carcinoma, and to analyze correlation between MMP-9 and invasion and metastasis of colorectal carcinoma. Methods 99 cases of colorectal carcinoma patients received radical resection in the Second Affiliated Hospital of Nanjing Medical University and Yancheng NO.3 People＇s Hospital from January 2007 to December 2010 were enrolled in our study. The expressions of MMP-9 in colorectal carcinoma and normal colorectal tissues were detected by using tissue chip technology combined with immunohistochemistry SP assay. The relations between the expressions of MMP-9 and clinicopathological features of colorectal carcinoma were analyzed. Results In colorectal carcinoma, the expression levels of MMP-9 was significantly higher than those of normal colorectal tissues（P〈0.001）. Patients with high stage of invasive depth, lymph node metastasis, and high linical stage showed obvious higher positive rates of MMP-9 than those of patients with low stage of invasive depth, without lymph node metastasis, and with low linical stage（P〈0.05）. It showed obvious negative correlation among MMP-9 and survival of patients with colorectal carcinoma. Patients with higher MMP-9 expression level in carcinoma tissues showed higher death rates（P〈0.05）. Conclusion The expression of MMP-9 in colorectal carcinoma is closely related to the depth of tumor invasion, lymph node metastasis. MMP-9 can relex the progress of colorectal carcinoma, and judge the prognosis of patients with colorectal carcinoma.
出处
《中国医药科学》
2016年第21期33-37,共5页
China Medicine And Pharmacy
基金
江苏省高校自然科学研究面上项目(15KJB320006)
关键词
结直肠癌
MMP-9
临床意义
Colorectal carcinoma
MMP-9
Clinical significance
