摘要
目的:观察小续命汤对APP/PS1转基因鼠海马区Aβ及GFAP蛋白的影响,探讨小续命汤改善认知功能障碍的作用机制。方法:采用APP/PS1双转基因小鼠建立阿尔茨海默病模型,将同月龄的同窝阴性鼠作为空白对照组,按照随机数字表将基因鼠分为模型组和小续命汤组,模型组和空白对照组不给药,小续命汤组按照13 g/kg连续灌胃90 d。Morris水迷宫法检测小鼠学习记忆能力,免疫组化染色观察3组小鼠大脑海马区Aβ、GFAP蛋白的表达。结果:与模型组比较,小续命汤组在水迷宫空间探索实验中,到达目标区域的时间及搜索距离明显缩短(P<0.05),穿梭次数明显增多(P<0.05);免疫组化结果显示:与空白对照组比较,模型组小鼠大脑海马区Aβ阳性蛋白及GFAP阳性细胞均明显增加(P<0.01);与模型组比较,小续命汤组Aβ蛋白的沉积及GFAP阳性细胞均明显减少(P<0.05)。结论:小续命汤改善APP/PS1基因鼠的认知功能可能与减少大脑海马区Aβ沉积、降低星形胶质细胞的活化有关。
Objective：To observe the effect of Xiaoxuming decoction （XXMD） on the expression of Aβ and GFAP protein in the hippocampus of APP/PS1 transgenic model mouse, and to explore its mechanism. Methods： APP/PS1 double transgenic mice were used to establish the Alzheimer＇s disease （AD） model, and the same month old littermates negative mice were chose as the control group. Transgenic mice were divided into the model group and the Xiao Xu Ming group by a random number table ,the control group and the model group didn＇t receive medicine treatment. Xiao Xu Ming group received XXMD at a dose of 13 g/kg for 90 days. Morris water maze experiment was used to observe the learning and memory ability of mice, and immunohistochemical staining was used to observe the expression of Aβ and GFAP proteins in the hippocampus of three groups. Results：Compared with the model group, the time of arrival in the target area and search distance in the Xiao Xu Ming group were significantly shortened （P〈 0.05 ）, and the times across platform were significantly increased （P〈0.05）. Immunohistochemical results showed that compared with wild group,both of Aβ protein deposition and GFAP positive cells were increased significantly in hippocampus of the transgenic group （P〈 0.01 ）. Compared with the model group, the Aβ protein deposition and GFAP positive cells were decreased in the Xiao Xu Ming group （P〈 0.05 ）. Conclusion ：XXMD can improve the cognitive function of APP/PS 1 transgenic mice, which may be associated with down-regulation of Aβ and GFAP expression in hippocampus.
作者
毛敬洁
陈亚萍
林如辉
李钻芳
朱晓勤
MAO Jingjie CHEN Yaping LIN Ruhui LI Zuanfang ZHU Xiaoqin(Fujian Academy oflntegrative Medicine ,Fujian University of Traditional Chinese Medicine ,Fuzhou,Fujian 350122,China Anxi Maternal and Child Health Hospital ,Anxi, Fujian 362400, China)
出处
《康复学报》
2016年第4期28-33,共6页
Rehabilitation Medicine
基金
福建省自然科学基金项目(2015J01335
2016J01397)
福建省中西医结合老年病重点实验室(福建中医药大学)开放课题(2008J1004)
福建中医药大学校管-科研创新平台专项课题(X2015023)
