摘要
设计完成了一体式微弱荧光检测器,该检测器可以检测经激发光照射后荧光染料、绿色荧光蛋白等物质发出的微弱荧光信号,由此测得荧光素、绿色荧光蛋白等物质的含量。检测器由光源模块、PN放大模块、采样控制模块和通讯模块组成。应用测试结果表明该检测器能检测1 000个光子的微弱荧光强度变化,精度上可检测3.0 pmol的荧光素,满足一般的微弱荧光检测需求。
A integrated detector is designed and accomplished for weak fluorescence signals,aiming at the detectionof the fluorescence intensity emitted by Fluorescent dyes,Green fluorescent protein or other material. The detectorcan be used to measure the concentration of the Fluorescent dyes,Green fluorescent protein or other material. Thedetector can be made up of several modules,which are the source of light,the amplifier of PN,sampling control unitand the communication unit. Application of the test results show that the detector can detect weak fluorescence inten-sity of 1 000 photons change,3.0 pmol Fluorescein. It can be used for general needs of weak fluorescence detection.photoelectric detectionintegratedfluorescence analysisconventional transimpedance amplifierinte-
出处
《电子器件》
CAS
北大核心
2016年第5期1129-1134,共6页
Chinese Journal of Electron Devices
基金
国家重大科学仪器设备开发专项目(2012YQ15008705)
浙江省科技计划项目(2014F10059)
关键词
光电检测
一体式
荧光分析
跨阻放大电路
积分放大电路
photoelectric detection
integrated
fluorescence analysis
conventional transimpedance amplifier
integrating transimpedance amplifier
