摘要
植物发育相关基因ARGOS基因受生长素诱导,在植物的生长发育和激素调控方面起着重要的作用。本实验选取茶树品种‘龙井43’作为试验材料,利用RT-PCR方法,从茶树c DNA中克隆得到茶树ARGOS基因,命名为Cs ARGOS。序列分析表明,Cs ARGOS基因开放阅读框为366 bp,编码121个氨基酸。进化树分析显示,Cs ARGOS蛋白与双子叶植物的进化树关系相对较近,与单子叶植物的进化树关系相对较远。序列多重比对显示,Cs ARGOS蛋白具有ARGOS蛋白的特征基序,C端均含有丰富的亮氨酸,N端亲水性氨基酸居多,这些区域在物种中是相对保守的。氨基酸组分、理化性质、亲水性/疏水性、二级结构预测和无序化分析显示,茶树Cs ARGOS是疏水性蛋白,以随机卷曲为主,无序化特征不明显。利用实时荧光定量PCR对Cs ARGOS基因在茶树中的表达进行检测,分析结果表明在不同组织中,Cs ARGOS基因的表达水平从高到低依次为根>第三叶>第四叶>老叶>嫩茎>第二叶>第一叶;不同激素处理下,Cs ARGOS的表达水平从高到低依次为GA_3>SA>ABA>Me JA>IAA。
ARGOS gene is induced by auxin and plays important roles in plant development and hormonal regulation. In this study,a gene encoding ARGOS-like protein was cloned by RT-PCR method using a c DNA template from tea plant（Camellia sinensis） cultivar ‘Longjing43’. This gene is named as Cs ARGOS. Sequence analysis showed that the open reading frame of Cs ARGOS gene has 366 bp length,which encoding 121 amino acids. Analysis of phylogenetic tree showed that Cs ARGOS protein has high similarity with dicotyledon plants and has low similarity with the monocotyledon plant. Multiple alignments showed that Cs ARGOS protein contains Leu-rich domain in the C-terminal and rich hydrophilic amino acids in N-terminal,which are the characterized motifs of ARGOS-type protein. Amino acid composition,physical and chemical properties,hydrophilicity/hydrophobicity,secondary structure predicted and disordered residues of Cs ARGOS protein were analyzed. The disordered residues of Cs ARGOS protein are not obvious. Most amino acids of Cs ARGOS protein are hydrophobic. The secondary structure of Cs ARGOS is mainly random coil. The expression levels of Cs ARGOS gene in tea plant were detected using quantitative real-time PCR analysis. In different tissues,the successive order of Cs ARGOS gene expression levels was root,the third leaf,the fourth leaf,old leaf,stem,the second leaf,and the first leaf. In different hormonal treatments,the successive order of Cs ARGOS gene expression levels was GA3,SA,ABA,Me JA,and IAA.
出处
《植物生理学报》
CAS
CSCD
北大核心
2017年第1期38-44,共7页
Plant Physiology Journal
基金
国家自然科学基金(31570691)~~
关键词
茶树
ARGOS
进化树分析
不同组织
激素处理
Camellia sinensis
ARGOS
phylogenetic tree analysis
different tissues
hormonal treatment
