摘要
目的基于双波长还原检测法构建微型化血液中CO检测专用仪。方法装置以发光二极管(LED)为光源,结合半透半反镜对LED光强进行校正,聚醚醚酮(PEEK)模块制作检测通路,辅以光电转换、数据处理、结果显示等组件。结果整机重量仅为540 g,尺寸为14 cm×12 cm×6.3 cm;检测池体积小于10μL,总样品消耗量小于400μL,有效光程10 mm,检测时间小于5分钟。结论可满足临床及刑侦对血液中CO含量现场快速检测的需求。
Objective A miniature instrument for determination of CO in blood based on dual wavelength sodium dithionite reduction method was constructed. Methods A LED lamp was used as light source and the light intensity was adjusted by combining semi transparent mirror into optical path. The optical path was made of poly ether ether ketone（ PEEK）, supported by other components for photoelectric conversion, data processing and results display. Results The whole device is lighter than 540 g with effective detection length of 10 mm and the total sample consumption is less than 400 μL. The detection time is shorter than 5 min. Conclusion The developed device can meet the clinic and forensic needs for fast and in field determination of CO in blood sample.
出处
《生命科学仪器》
2016年第5期60-63,共4页
Life Science Instruments
基金
公安部重点研究计划(201202ZDYJ005)
关键词
发光二极管
微型
CO检测
血液
light-emitting diode
miniature
determination of CO
blood
