摘要
目的评价脐带血间充质干细胞输注对半相合移植患者急性移植物抗宿主病（aGVHD）的预防效果。方法收集18例患者接受半相合移植后不同阶段的血清样品，用酶联免疫吸附试验法检测血清三种细胞因子血清白细胞介素-6、干扰素-γ、肿瘤坏死因子-α的水平变化，记录半相合移植后急性GVHD的发生状况。结果18例患者中发生aGVHD 4例，Ⅱ度以下aGVHD 2例，Ⅲ～Ⅳ度2例，累计aGVHD发生率为22.2%，重度aGVHD发生率为11.1%。与未发生GVHD的患者组比较，三种细胞因子水平在GVHD组显著升高。结论间充质干细胞输注可以减少半相合移植后GVHD的发生率和严重程度，其机制可能与间充质干细胞减少细胞因子的血清水平有关。
ObjectiveTo evaluate the prevention effect of mesenchymal stem cells from umbilical cord treatment on acute graft-versus-host disease （GVHD） after haplo-identical hematopoietic stem cell transplantation.MethodsEighteen serum sample was collected from patients who received haplo-identical hematopoietic stem cell transplantation at different points after transplants, the levels of tumor necrosis factor-α, interleukin-6, interferon-γ were detected by ELISA, the incidence of acute GVHD was recorded.ResultsFour cases of acute GVHD was recorded among in 18 patients, two cases was in degree Ⅱand two cases was in degree Ⅲ-Ⅳ with acute GVHD, the total incidence of acute GVHD was 22.2% and serious GVHD was 11.1%. Compared with patients without acute GVHD, the level of three cytokines in the patients with acute GVHD was increased.ConclusionsMesenchymal stem cells treatment can reduce the incidence and severity of acute GVHD of the patients received haplo-identical hematopoietic stem cell transplantation, the mechanism of mesenchymal stem cells treatments may be related to inhibition of cytokines in serum.
作者
林剑波
林进宗
鹿全意
Lin Jianbo Lin Jinzong Lu Quanyi(Department of Hematology, Xiehe Clinical Medicine College of Fujian Medical University, Fuzhou 350000, China Zhongshan Hospital of Xiamen University, Xiamen 361004, China)
出处
《中国实用医刊》
2017年第1期13-15,共3页
Chinese Journal of Practical Medicine
基金
福建省科技重点项目（2012D065）
关键词
间充质干细胞
半相合移植
移植物抗宿主病
细胞因子
Mesenchymal stem sells
Haplo-identical hematopoietic stem cell transplantation
Graft-versus-host disease
Cytokines
