摘要
目的建立氧化应激损伤模型,研究过氧化氢(H202)对内皮细胞增殖活性的影响,为深入探讨氧化应激致血管内皮细胞损伤机制奠定基础。方法以0、50、100、150、300、400和600μmol/L H202作用于人脐静脉内皮细胞(HUVEC),在8、12和24h时间段内采用MTT法测定细胞的增殖活性。结果 50μmol/L H_2O_2作用8h对HUVEC活性无影响,与对照组比较,差异无统计学意义(P>0.05);其余各浓度各时间段内,H_2O_2均可引起细胞活性降低,与对照组比较,差异有统计学意义(P<0.01)。各两组比较,8h组与12h组、24h组比较,差异有统计学意义(P<0.05);12h组与24h组比较,差异无统计学意义(P>0.05)。12h的IC_(50)为133.3μmol/L。结论在一定浓度时间范围内,H202对HUVEC有增殖抑制作用,150μmol/L H_2O_2处理12h为最佳实验浓度时间。
Objective To establish oxidative stress injury model and investigate the effect of hydrogen peroxide on proliferation of endothelial cells, in order to lay a foundation for further research on the mechanism of oxidative stress induced damage to vascular endothe- lial ceils. Methods Human umbilical vein endothelial ceils（HUVEC） were induced by 0,50,100,15Q ,300,400 and 600μmol/L H202, and MTT assay was use to determine the proliferation of endothelial cells in 8h,12h and 24h. Results There was no effect on HUVEC cells activity after induced by 50μmol/L H2O2 for 8h, compared to the control group, and the difference was not statistically significant（P 〉 0.05 ）. H2 02 could cause HUVEC ceils activity decreased with other concentration of each time, compared to the control group, and the difference was great statistically significant（P 〈 0.01 ）. In multiple comparisons, the difference between 8h group and 12h group,24h group was statistically significant（P 〈 0.05） ; the difference between 12h group and 24h group was not statistically significant（ P 〉 0.05）. The IC50 of 12h was 133.3μmol/L. Conclusion In a certain time and concentration range, H202 had proliferative effect on HUVEC cells. The best experimental H2 02concentration and time were 150μmol/L and 12h.
出处
《医学研究杂志》
2017年第1期46-48,共3页
Journal of Medical Research
基金
国家自然科学基金资助项目(81260290)
关键词
过氧化氢
浓度
内皮细胞
增殖
Hydrogen peroxide
Concentrations
Endothelial ceils
Proliferation
