摘要
Cocktail探针药物法作为一种快速、准确、高通量的评价CYP450酶活性的研究方法广泛应用于药物研发早期筛选、药物代谢途径及机制研究、药物相互作用研究、指导临床合理用药、上市药物再评价及协助肝肾病理研究等诸多领域。该文对Cocktail探针药物法的特点做简要说明,着重介绍其在中医药领域内的应用进展,从中药活性成分的代谢机制研究,炮制过程,方剂配伍对CYP450的影响,揭示中成药CYP450代谢特点规律,民族药学应用情况,中药保肝作用及中医证候模型研究等,并总结归纳现有研究成果,比较其在中西医药领域内应用的异同点,为该领域的深入研究工作提供参考。
Cocktail probe substrates approach is a fast, sensitive and high through put method to determine cytochrome P450 en- zymes activity. It has been widely used to screen early drug development, analyze drug metabolism types and confirm the metabolism pathways, study drug-drug interactions, optimize clinical regimen, evaluate post marketing drugs and help liver/kidney pathological studies. This article reviewed characteristics of Cocktail probe substrates, focused on the application to traditional Chinese medicine to CYP450 system as follows： the metabolic pathway research of Chinese herb active ingredients; processing way and compatibility of medical herbs affect CYP450 ; find out the metabolic characteristic of Chinese patent medicine, study in pharmacy of national minority ; do research in liver protective effect of traditional Chinese medicine and evaluate traditional Chinese medicine syndromes in animal models. This article make a summary of existing research results and also make a comparison of cocktail probe substrates approach ap- plication to western medicine and Chinese medicine.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第24期4541-4549,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81273936
81303141
81373890)
教育部"创新团队发展计划"项目(IRT_14R41)
