摘要
目的研究胃癌的高频变异位点并探讨其与胃癌临床病理特征的关系。方法通过二代测序平台对26例胃癌组织进行靶向深度测序,与人类基因组UCSC hg19数据库比对,筛选出胃癌中的高频变异位点,并对临床病理特征进行分析。结果胃癌中突变率较高的基因位点有TTN rs12693166(15/26)、rs2627043(22/26),ALK rs3795850(19/26)、rs1881420(23/26),MUC2 rs2856111(20/26)、rs56365200(20/26)。高频变异位点与胃癌的年龄、性别、分化程度、肿瘤浸润深度、淋巴结转移、TNM分期以及劳伦斯分型等临床病理特征之间差异无统计学意义。结论 TTN rs12693166、rs2627043、ALK rs3795850、rs1881420、MUC2 rs2856111、rs56365200位点在胃癌中变异频率较高,可能与胃癌的发生存在一定的相关性。未发现高频变异位点与胃癌患者的临床病理特征存在明显的相关性。
Objective To study the recurrent mutation sites in gastric cancer and explore its relation to clinicopathologic significance of gastric cancer. Methods New generation sequencing was applied to 26 cases of gastric cancer tissues by the targeted deep sequencing. The sequencing data were compared with UCSC hg19 database, and the recurrent mutation sites were screened and analyzed with the clinicopathologic features of gastric cancer. Results The recurrent mutation sites in gastric cancer were TTN rs12693166 （15/26）, TTN rs2627043（22/26）, ALK rs3795850（19/26）, ALK rs1881420（23/26）, MUC2 rs2856111 （20/26 ） , MUC2 rs56365200 （ 20/26 ） . There were no statistical significance between these recurrent mutation sites and the clinicopathological features in patients with gastric cancer. Conclusion The recurrent mutation sites （ TTN rs12693166 , rs2627043 , ALK rs3795850 , rs1881420 , MUC2 rs2856111 , rs56365200） might be correlated with the carcinogenesis of gastric cancer. No significant association were found between these recurrent mutation sites and the clinicopathological features in patients with gastric cancer.
出处
《河南科技大学学报（医学版）》
2016年第4期255-258,共4页
Journal of Henan University of Science & Technology:Medical Science
关键词
胃癌
测序分析
高频变异位点
临床病理特征
gastric cancer
sequencing analysis
recurrent mutation sites
clinicopathological characteristics
