摘要
目的观察二甲双胍对人绒毛膜癌细胞凋亡的影响和可能的作用机制。方法选用人绒毛膜癌细胞系JEG-3,分为对照组和二甲双胍组(终浓度为5、10、20、40mmol/L)处理48h后,使用流式细胞术和免疫荧光检测细胞凋亡情况,分别采用实时荧光定量聚合酶链反应(PCR)和免疫蛋白印迹检测凋亡相关基因半胱天冬酶-3(Caspase-3)、Bcl-2和凋亡调节蛋白(Bax)的mRNA及蛋白变化趋势。结果和对照组相比,二甲双胍组的JEG-3细胞早晚期凋亡率显著上升,同时Caspase-3和Bax的mRNA及蛋白表达水平显著增加,但Bcl-2的mRNA和蛋白表达显著降低。结论二甲双胍可以通过Caspase-3及Bcl-2/Bax通路诱导JEG-3细胞发生凋亡。
Objective To investigate the effects of metformin on apoptosis of human choriocarcinoma cell and its possible action mechanism.Methods The human choriocarcinoma cell line JEG-3was selected and divided into the control group and metformin groups（final concentrations of 5,10,20,40mmol/L）.Flow cytometry and immunofluorescence assay were adopted to detect cell apoptosis at 48 hafter treatment.The mRNA and protein change trend of Caspase-3,Bcl-2and Bax were measured by Real-time PCR and Western Bolt.Results Compared with the control group,the early and late apoptosis rate of JEG-3cells in the metefomin groups were remarkably increased,meanwhile mRNA and protein expression levels of Caspase-3and Bax were significantly increased,but Bcl-2mRNA and protein expression were significantly decreased.Conclusion Metformin induces the JEG-3cells to generate apoptosis by the Caspase-3and Bcl-2/Bax pathways.
出处
《国际检验医学杂志》
CAS
2016年第23期3280-3282,共3页
International Journal of Laboratory Medicine
