摘要
目的：建立护肝宁丸中虎杖苷、槲皮素和丹参酮ⅡA的测定方法。方法：采用PhenomenexLunaC18色谱柱（4.6mm×250mm,5μm）;流动相为甲醇-0.1%磷酸溶液,梯度洗脱;体积流量1.0m L·min-1;柱温30℃;检测波长306nm（0-20min）,360nm（20-59min）,270nm（59-69min）。结果：虎杖苷、槲皮素、丹参酮ⅡA分别在0.146-1.46μg（r=0.9993）、0.03504-0.3504μg（r=0.9995）、0.03004-0.3004μg（r=0.9996）范围内线性关系良好,平均加样回收率分别为95.9%、96.9%、96.2%,RSD分别为0.87%、1.32%、0.60%。结论：检测3种成分的HPLC法优于只测定虎杖苷的护肝宁丸现执行标准。
Objective：To establish method for the determination of polydatin, quercetin, tanshinone H A in Hugan- ning Pills. Methods ：The analysis was carried out on Phenomenex Luna C ls column （4.6mm x250mm,5 μ m ）. The mobile phase consisted of methanol-0.1% phosphoric acid solution with step gradient elution. The flow rate was 1.0 m L·min-1, the column temperature was 30℃;The detection wavelengths were 306nm, 360nm and 270nm, respec- tively. Results ：The experiments showed a good linearity in the range of 0.146-1.46 μg （ r=0.9993 ）for Polydatin, 0.03504-0.3504μg （ r=0.9995 ） for Quercetin, 0.03004-0.3004 μg （ r=0.9996 ） for Tanshinone II A, respectively. The average recoveries of the method was 95.9%,96.9%,96.2%, respectively. The RSD was 0.87%, 1.32%,0.60%, respectively. Conclusion： HPLC method of determining three constituents is superior to the current method which is only used for the determination of polydatin in Huganning pills
出处
《中医药临床杂志》
2016年第10期1479-1482,共4页
Clinical Journal of Traditional Chinese Medicine
