摘要
目的 了解胶原蛋白肽（CP）对X射线照射小鼠免疫功能的调节作用。方法 ICR小鼠按体重分层随机分为5组，分别为健康对照组、2 Gy、5 Gy、2 Gy＋CP 600 mg·kg-1·d-1组（2 Gy CP）和5 Gy＋CP 600 mg·kg-1·d-1组（5 Gy CP）。6 MV X射线单次全身照射诱导免疫抑制小鼠模型，剂量率为6 Gy/min。2 Gy CP和5 Gy CP组的小鼠连续7 d腹腔注射给予胶原蛋白肽600 mg/kg。测量小鼠体质量、胸腺和脾脏质量，计算胸腺和脾脏指数；检测刀豆蛋白A（ConA）诱导的脾脏T淋巴细胞增殖能力及脾脏T淋巴细胞分泌的白介素-2（IL-2）的水平。结果 与健康对照组相比，X射线照射小鼠的胸腺指数、脾脏指数、脾脏T 淋巴细胞增殖能力及IL-2浓度显著降低（F=76.857、181.870、63.133、8.499，P〈0.05）。腹腔注射胶原蛋白肽后，与同等剂量单纯照射小鼠比较，上述指标均有所恢复。结论 腹腔注射胶原蛋白肽能够增强X射线照射小鼠的免疫功能。
Objective To study the effects of collagen peptides（CP） on the immune functions of X-ray irradiated mice. Methods ICE mice were randomly divided into five groups according to body weight：control, 2 Gy, 5 Gy, 2 Gy CP （2 Gy X-ray irradiation ＆ administrated with collagen peptides 600 mg·kg-1·d-1） and 5 Gy CP （5 Gy X-ray irradiation ＆ administrated with collagen peptides 600 mg·kg-1·d-1） groups. Immunosuppressant mouse model was established by iradiating ICE mice with 6 MV of X-rays generated by a linear accelerator, where the whole body of the mice was irradiated with a single dose of 2 Gy or 5 Gy at dose rate of 6 Gy/min. And the mice in groups of 2 and 5 Gy CP were injected intraperitoneally with collagen peptides （600 mg·kg-1·d-1） for 7 days after irradiation. On the day 8, the mice were decapitated. The body weights, thymus and spleen indexes, IL-2 level and proliferations of spleen T lymphocytes induced by ConA were detected. Results Compared with the control group, the thymus and spleen indexes, the level of IL-2 and proliferations of spleen T lymphocytes were significantly decreased（F=76.857, 181.870, 63.133, 8.499, P〈0.05）. These parameters were restored to some extent in mice injected with collagen peptides when compared with the mice irradiated with same dose of X-rays. Conclusion Peritoneal injection of collagen peptides can enhance the immune functions in X-ray irradiated mice.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2016年第11期811-814,共4页
Chinese Journal of Radiological Medicine and Protection
