摘要
目的检测慢性阻塞性肺疾病(简称慢阻肺)稳定期患者血清氧化应激反应标志物丙二醛(MDA)、蛋白质羰基(PC)、8-羟基脱氧鸟苷(8-OHd G)、总抗氧化能力(TAC)水平,探讨氧化/抗氧化失衡在慢阻肺发病中的作用。方法选取200例慢阻肺稳定期患者(慢阻肺组)和100名体检健康者(对照组)。检测血清MDA、PC、8-OHd G和TAC的含量;同时对受试者进行肺功能检测,收集一般资料。慢阻肺组按是否吸烟分为吸烟亚组和未吸烟亚组,按气流受限程度分为轻-中度亚组和重-极重度亚组。结果与对照组比较,慢阻肺组患者血清MDA、PC、8-OHd G浓度明显升高(P均<0.01),而血清TAC水平显著降低(P均<0.01)。吸烟亚组慢阻肺患者血清PC、8-OHd G浓度明显高于未吸烟亚组的慢阻肺患者(P均<0.05)。重-极重度亚组的慢阻肺患者血清PC的浓度明显高于轻-中度亚组的慢阻肺患者(P<0.01)。多元线性回归分析显示:慢阻肺患者血清PC、8-OHd G的浓度与FEV_1%pred存在关联,血清中PC、8-OHd G的水平均与FEV_1%pred呈负相关,且血清PC的水平对FEV_1%pred的影响比8-OHd G大(β=-0.230,-0.219,P均<0.01)。结论吸烟引起慢阻肺患者血清PC、8-OHd G的水平异常升高,血清PC、8-OHd G的水平均与FEV_1%pred呈负相关,表明氧化应激在慢阻肺的发病过程中起了重要作用。
Objective To detect the level of oxidative stress markers in serum,including malondialdehyde（ MDA）,protein carbonyls（ PC）,8-hydroxy-2＇-deoxyguanosine（ 8-OHd G） and total antioxidant capacity（ TAC）,in patients with stable chronic obstructive pulmonary disease（ COPD）,and explore the impacts of oxidant / antioxidant imbalance in pathogenesis of COPD. Methods Two hundred stable COPD patients（ the COPD group） and 100 healthy individuals（ the control group） were recruited in the study. The concentrations of MDA,PC,8-OHd G and TAC in serum were detected. Pulmonary function test was performed and the general informations for each subjects were collected. The COPD patients were divided into a smoking subgroup and a non-smoking subgroup,or divided into a mild-moderate airflow limitation subgroup and a severe-extremely severe airflow limitation subgroup. Results The levels of serum MDA,PC and 8-OHd G in the COPD group were significantly higher than those in the control group（ all P〈0. 01）,but the level of serum TAC was significantly lower than that in the control group（ P〈0. 01）. In the COPD patients,the levels of PC and 8-OHd G in the smoking subgroup were significantly higher than those inthe non-smoking subgroup（ both P〈0. 05）. The level of PC in the severe-extremely severe airflow limitation subgroup was significantly higher when compared with the mild-moderate airflow limitation subgroup（ P〈0. 01）. Multiple linear regression analysis showed that the levels of PC and 8-OHdG were negatively related with FEV1% pred in the COPD patients,and the PC had greater impacts than 8-OHdG（ β =- 0. 230,- 0. 219,P〈0. 01）. Conclusion Smoking can induce the abnormal increase of PC and8-OHdG in serum which are negatively related with FEV1% pred in COPD patients,which suggests that oxidative stress might play an important role in pathogenesis of COPD.
作者
蒋雪莲
钟萍
黄成亮
孟原竹
何芳
范贤明
Jiang Xuelian Zhong Ping Huang Chengliang Meng Yuanzhu He Fang Fan Xianming.(Second Department of Respiratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, 646000, Chin)
出处
《中国呼吸与危重监护杂志》
CAS
北大核心
2016年第6期542-547,共6页
Chinese Journal of Respiratory and Critical Care Medicine
基金
四川省科学技术厅-泸州市人民政府-泸州医学院联合科研专项资金计划项目(编号:14ZC0048)
四川省教育厅重点项目(编号:16ZA0198)
关键词
慢性阻塞性肺疾病
氧化应激
丙二醛
蛋白质羰基
8-羟基脱氧鸟苷
总抗氧化能力
Chronic obstructive pulmonary disease
Oxidative stress
Malondialdehyde
Protein carbonyls
8-hydroxy-2＇-deoxyguanosine
Total antioxidant capacity
