摘要
目的:通过卡铂诱导卵巢癌耐药的细胞系对免疫细胞功能影响的研究,从免疫学角度探讨耐药卵巢癌转移或复发的机制。方法:建立卵巢癌SKOV3细胞对卡铂耐药的细胞系SKOV3/CBP,ELISA法检测SKOV3和SKOV3/CBP细胞吲哚2,3双加氧酶(IDO)表达;将两组细胞分别与淋巴细胞、NK细胞及C8^+T细胞培养,MTT法检测淋巴细胞的增殖能力、LDH检测NK细胞的杀伤能力及ELISPOT检测C8^+T细胞的杀伤能力。结果:与SKOV3细胞株相比,耐药细胞株SKOV3/CBP内IDO表达明显增强(P<0.05),且与其共培养的淋巴细胞的增殖能力、NK细胞的杀伤能力及CD8+T细胞的杀伤能力较SKOV3组降低,差异有统计学意义(P<0.05)。结论:卡铂耐药SKOV3细胞内IDO表达增加,其可通过降低免疫细胞作用的敏感性而发生免疫逃逸促进耐药肿瘤的转移和复发,IDO可作为卵巢癌卡铂耐药治疗新的靶点。
Objective： Study on the mechanism of resistant ovarian cancer metastasis or recurrence from the perspective of immunology by carboplatin induced multidrug resistance in ovarian carcinoma cell line effect on cellular immune function. Methods： The resistant SKOV3 /CBP cell lines were established by carboplatin inducing human ovarian cancer SKOV3 cells. Indole 2,3-dioxygenase（ IDO） expressions of SKOV3 and SKOV3/CBP cells were detected by ELISA method. Then the two groups of cells were cultured with lymphocytes,NK cells and C8＋T cells separately,lymphocyte proliferation were monitored by MTT method,the killing ability of NK cell were tested by LDH release assay,the function of C8＋T cells were detected by ELISPOT assay. Results： Compared with SKOV3 cell lines,the expression of IDO was significantly increased in SKOV3/CBP cell lines（ P〈0. 05）,and the proliferation ability of lymphocyte,NK cell killing capacity and CD8~＋T cell killing ability of SKOV3/CBP cells group were significantly decreased（ P〈0. 05） than that of SKOV3 cells group. Conclusion： SKOV3/CBP cells that increased expression of IDO can reduce the sensitivity of the immune cells and immune escape,promote metastasis and relapse of chemotherapy resistant tumors further. IDO can be new promising targets for treatment of carboplatin resistance of ovarian carcinoma.
出处
《现代妇产科进展》
CSCD
北大核心
2016年第10期744-746,共3页
Progress in Obstetrics and Gynecology
基金
山西省自然基金资助项目(No:2013011045-5)
关键词
卵巢癌
卡铂
耐药性
IDO
免疫功能
Ovarian cancer
Carboplatin
Chemotherapy resistance
IDO
Immune function
