摘要
目的观察miR-21在人膀胱癌5637细胞中的表达变化,探讨其对细胞增殖和侵袭的影响。方法用RT-PCR技术检测膀胱癌5637细胞和人正常膀胱上皮细胞中的miR-21表达。将培养好的5637细胞随机分为转染组、阴性对照组、空白对照组,前两组分别转染AS-miR-21(沉默miR-21)、无效寡核苷酸序列,空白对照组不做任何处理;分别于基因沉默后24、48、72 h采用CCK-8法测算细胞增殖指数,基因沉默后24 h用Transwell侵袭实验检测细胞侵袭能力。结果与正常膀胱上皮细胞相比,人膀胱癌5637细胞中miR-21表达上调;沉默miR-21后,转染组细胞增殖指数、侵袭能力低于阴性对照组、空白对照组(P均<0.05)。结论 miR-21在人膀胱癌5637细胞中高表达,沉默miR-21基因后膀胱癌细胞的增殖能力和侵袭性降低。
Objective To investigate the expression changes of miR-21 in human bladder cancer 5637 cells and its effect on cell proliferation and invasion. Methods The expression of miR-21 in the bladder cancer 5637 cells and human urothelial cells was detected by RT-PCR. The cultured 5637 cells in the logarithmic phase were randomly divided into the transfected group,negative control group and blank control group. The transfected group and negative control group were transfected with AS-miR-21（ silencing miR-21） and invalid oligonucleotide sequence,respectively,and the blank control group was not treated. The cell proliferation was respectively detected by CCK-8 assay at 24,48,72 h after gene silencing,and the cell invasion was evaluated by Transwell invasion assay at 24 h. Results The expression of miR-21 in 5637 cells was higher than that of the urothelial cells. Compared with the negative control group and blank control group,the proliferation and invasion abilities of the transfected group were significantly decreased after silencing miR-21（ all P〈0. 05）. Conclusion miR-21 is overexpressed in human bladder cancer 5637 cells,and silencing miR-21 can inhibit the proliferation and invasion of bladder cancer 5637 cells.
作者
李振辉
韩俊岭
师磊
宋东奎
LI Zhenhui HAN Junling SHI Lei SONG Dongkui(The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
出处
《山东医药》
CAS
北大核心
2016年第37期8-10,共3页
Shandong Medical Journal
基金
国家自然科学基金面上项目(81272823)
河南省教育厅科技创新团队支持计划(14IRTSTHN020)
