摘要
为筛选高效脱除玉米赤霉烯酮(Zearalenone,ZEN)的脱毒菌株,利用96孔板高通量筛选法,从135份赤霉病高发区域土壤样品中成功筛选出具有较强ZEN清除作用的两株芽孢杆菌Fu2-3和Fu41-3。经16S rRNA序列分析,菌株Fu2-3和Fu41-3分别为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)和特基拉芽孢杆菌(Bacillus tequilensis)。对其降解机理的初步研究表明,两株菌对ZEN均具有一定程度的吸附作用,但主要脱毒作用仍为生物的降解作用。BLYES法研究结果表明,菌株Fu41-3的脱毒体系中仍具有较大雌激素毒性,菌株Fu2-3脱毒体系无雌激素响应,该菌可将ZEN转化为雌激素含量极低的代谢产物,脱毒效果更佳。筛选获得了两株ZEN降解芽孢杆菌,为ZEN的生物脱毒提供了一定的理论依据。
The study was aimed to screen the strain for efficiently detoxifying zearalenone （ZEN）. Two strains named Fu2 - 3 and Fu41 - 3 with good performance in scavenging ZEN were screened out by 96 - well plate high throughput screening among 135 soil samples collected from different areas where were highly infected by gibberellic disease. The strains were preliminarily identified as Bacillus amyloliquefa- ciens and Bacillus tequilensis based on 16S rRNA gene sequencing. The preliminary study on their detoxi- fication mechanism showed that they were able to adsorb ZEN, but the main detoxification was bio - deg- radation. The result of BLYES assay indicated that the detoxification system of strain Fu41 -3 still con- tained considerable estrogen toxicity, while strain Fu2 -3 did not, by which ZEN would be transformed into metabolite with extremely low estrogenic toxicity. The two strains were isolated through screening, which provides theoretical evidence for bio - detoxification of ZEN.
作者
张倩
熊犍
赵晨
汪洋
张晓琳
ZHANG Qian XIONG Jian ZHAO Chen WANG Yang ZHANG Xiao -lin(College of Food Science and Engineering, South China University of Technology, Guangzhou Guangdong 510641 Academy of State Administration of Grain, Beijing 100037)
出处
《粮油食品科技》
2016年第6期76-81,共6页
Science and Technology of Cereals,Oils and Foods
基金
国家自然科学基金(31302007)
关键词
玉米赤霉烯酮
芽孢杆菌
生物脱毒
zearalenone
Bacillus subtilis
bio - detoxification
