摘要
目的分析肺炎克雷伯菌的临床分布情况及对常见药物的耐药程度,探讨碳青霉烯类抑制法用于检测产碳青霉烯酶肺炎克雷伯菌的应用价值。方法将258株肺炎克雷伯菌作为研究对象,采用法国梅里埃VITEK-2全自动微生物分析仪鉴定及药敏分析,WHONET 5.6软件分析数据,碳青霉烯类抑制法检测碳青霉烯酶。结果2015年医院分离出肺炎克雷伯菌主要来自痰液标本,占66.28%;碳青霉烯类耐药的肺炎克雷伯菌主要分离于ICU,占74.36%;亚胺培南、美罗培南、哌拉西林/他唑巴坦耐药率较低;碳青霉烯类抑制法能够特异、敏感地检测产碳青霉烯酶菌株。结论肺炎克雷伯菌在不同标本中的耐药率不同,临床抗菌治疗应综合分析,合理使用抗菌药物;利用碳青霉烯类抑制法可有效发现产酶菌株,在医院感染监控中有重要价值。
OBJECTIVE To investigate the clinical distribution of K lebsiella pneumoniae strains and analyze the drug resistance to the commonly used antibiotics and explore the application value of carbapenems inactivation method in detection of carbapenemase‐producing K .pneumoniae .METHODS A total of 258 strains of K .pneumon‐iae were recruited as the study objects ,then the strains were identified by using VITEK‐2 automatic microorgan‐ism analyzer ,the drug susceptibility testing was performed ,the data were analyzed with the use of WHONET 5 . 6 software ,and the carbapenemase was detected by means of carbapenems inactivation method .RESULTS The ma‐jority of the K .pneumoniae strains were isolated from the sputum specimens in 2015 ,accounting for 66 .28% ,and 74 .36% of the carbapenem‐resistant K .pneumoniae strains were isolated from ICU .The drug resistance rates to imipenem ,meropenem , and piperacillin‐tazobactam were relatively low . The carbapenemase‐producing strains could be specifically ,sensitively detected by the carbapenems inactivation method .CONCLUSION The K .pneu‐moniae strains isolated from the different specimens vary in the drug resistance rate;it is necessary for the hospital to comprehensively analyze the antimicrobial treatment and reasonably use antibiotics .The carbapenems inactiva‐tion method may facilitate the detection of the carbapenemase‐producing strains ,and it has significant value in the monitoring of nosocomial infections .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2016年第22期5053-5055,共3页
Chinese Journal of Nosocomiology
基金
河北省卫生厅重点科技研究计划基金资助项目(20150332)
关键词
肺炎克雷伯菌
碳青霉烯类抑制法
耐药性
Klebsiella pneumoniae
Carbapenems inactivation method
Drug resistance
