摘要
[目的]优化福林试剂法的反应条件。[方法]以从紫外诱变样品中筛选获得的1株高产藤仓赤霉菌(G.fujikuroi)F12为研究对象,采用福林试剂法,通过溶液吸光值的变化定量检测藤仓赤霉菌发酵液中赤霉酸(GA3)的产量。[结果]当福林试剂的用量为1.5 m L、盐酸用量为3.0 m L、加热时间为20 min时,所得溶液吸光值稳定性最好,在4 h以内基本保持不变。[结论]福林试剂法操作简便、检测快速准确,可用于藤仓赤霉菌发酵液中GA3的快速检测。
Objective] The aim was to optimize reaction conditions of Folin-Ciocalteu’s phenol reagent.[Method] With high-yield Gibberella fujikuroi F12 as the research strain which was isolated from large quantities of UV mutation samples, Folin-Ciocalteu’ s phenol reagent method was used to quickly detect the gibberellin acid （ GA3 ） yield in Gibberella fujikuroi fermentation broth by the change of absorbance.[ Result] When 1.5 mL Folin-Ciocalteu’s phenol reagent, 3.0 mL hydrochloric acid as well as 20 min heating time was selected, the final solution absor-bance had the best stability and still remained basically unchanged within 4 h.[Conclusion] The optimized method can quickly and accurately detect the GA3 yield in Gibberella fujikuroi fermentation broth.
出处
《安徽农业科学》
CAS
2016年第28期17-18,29,共3页
Journal of Anhui Agricultural Sciences
