摘要
目的采用气相色谱法同时测定八宝惊风散中薄荷脑、龙脑、丁香酚和麝香酮的含量。方法使用Supelcowax^(tm-10)毛细管柱(30 m×0.25 mm×0.25μm),检测器FID,采用程序升温,初始温度60℃,以15℃·min^(-1)的速率升温至230℃,保持5 min。结果在同一色谱条件下,薄荷脑、龙脑、丁香酚和麝香酮分别在0.51~3.06μg,98.60~591.63μg,19.74~118.44μg和0.96~5.77μg内与峰面积呈良好的线性关系;平均回收率分别为98.6%(RSD=2.3%),99.0%(RSD=2.5%),98.5%(RSD=1.9%),99.5%(RSD=2.2%)。结论该方法灵敏、准确、重复性好,可作为八宝惊风散的质量控制提供依据。
Objective To establish a gas chromatogram method to determine menthol, borneol, eugenol and musk ketone in Babao Jingfeng granules. Methods Supelcowax^（tm-10） capillary column（30 m×0.25 mm×0.25 μm） and FID detector were used. The temperature programming rose from 60 ℃ to 230 ℃ at 15 ℃·min^（-1）, lasting 5 min at the end. Results Under the same chromatogram condition, there was linearity among menthol, borneol, eugenol and musk ketone at 0.51-3.06 μg, 98.60-591.63 μg, 19.74-118.44 μg, and 0.96-5.77 μg, respectively. The average recovery was 98.6%（RSD = 2.3%）, 99.0%（RSD = 2.5%）, 98.5%（RSD= 1.9%）, 99.5%（RSD = 2.2%）. Conclusion This method is sensitive, accurate and reliable, which can be used applied to control the quality of Babao Jingfeng granules.
作者
杨宝
刘佳
陈繁华
邬伟魁
古炳明
YANG Bao LIU Jia CHEN Fan-hua WU Wei-kui GU Bing-ming(Meizhou Institute for Food and Drug Control, Meizhou Guangdong 514071)
出处
《中南药学》
CAS
2016年第10期1113-1116,共4页
Central South Pharmacy
关键词
八宝惊风散
气相色谱法
薄荷脑
龙脑
丁香酚
麝香酮
Babao Jingfeng granule
gas chromatogram
menthol
borneol
eugenol
musk ketone
