摘要
鸭细小病毒是新近发生的引起樱桃谷肉鸭短喙、长舌、发育迟缓的1种新型水禽细小病毒,目前尚无可靠的快速诊断方法。本研究根据鸭细小病毒的VP3基因设计了2对特异性引物,建立了套式PCR检测方法。该方法对鸭细小病毒DNA的最低检出量为100copies,不能扩增鹅细小病毒、番鸭细小病毒、鸭肠炎病毒等。采用该方法对采自山东、江苏、安徽等地的30份疑似鸭细小病毒感染病料进行检测,结果显示阳性率为27/30(90%),与病毒分离结果符合率为90%;而普通PCR的阳性检出率为8/30(26.7%)。上述结果表明,建立的套式PCR方法具有较高的敏感性和特异性,可用于鸭细小病毒的临床诊断和分子流行病学调查。
A novel waterfowl parvovirus which caused short bills with protruding tongues and growth retardation in Cherry Valley meat-type ducks in North China was isolated and identified. Till now,there are not any effective methods for detection of duck parvovirus（DPV）. Two pairs of specific primers based on VP3 genes of DPV were designed,and a nested PCR assay for DPV detection was established. The results showed that the sensitivity of the nested PCR was 10a times higher than that of general PCR assay and the minimum detectable amount was 100 copies. Duck parvovirus could be well amplified and goose parvovirus, muscovy duck parvovirus, duck enteritis virus and other waterfowl origin viruses could not be amplified using the nested PCR. Thirty clini- cal samples from Shandong,Jiangsu and Anhui provinces were detected and 27/30 were deter- mined to be DPV positive. All these data indicated that the nested PCR was a sensitive,specific and rapid diagnosis method for detecting duck parvovirus.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2016年第10期1706-1709,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31272583
31472199)
现代农业产业技术体系建设专项资金资助项目(CARS-43-10)
山东省科技发展计划资助项目(2014GNC111023)
关键词
鸭细小病毒
套式PCR
樱桃谷肉鸭
duck parvovirus
nested PCR
Cherry Valley meat-type ducks
