摘要
利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术分析渤海N油田注水和未注水油藏中细菌和古菌群落结构组成及分布特征,试分析注水过程对油藏微生物群落丰度和种群的影响,为开展目标油田本源微生物采油试验提供技术支持。结果显示,注水采油井中细菌丰度和种类明显高于未注水采油井,其中注水采油井中的细菌主要为固氮螺菌属(Azospira sp.)、铜绿假单胞菌(Pseudomonas sp.)、陶厄氏菌属(Thauera sp.);注水井古菌的丰度和种类与未注水井也存在一定差异,主要为热自养甲烷热杆菌(Methanothermobacter thermautotrophicus)、甲烷鬃毛菌属(Methanosaeta)、嗜泉古菌(Crenarchaeote)。注水井和未注水井中的细菌、古菌种类分布有限,但丰度较高,主要为提高原油采收率有益的采油菌种,显示该区块具备开展本源微生物微生物采油技术实施的条件。
Denaturing gradient gel electrophoresis （ DGGE ） method was used to analyze the structures of microorganism population in water injection wells and no-water injection wells of an oil reservoir in Bohai oil field N, and investigate the process effect of water injection to the abundance and population of microbial communities in the reservoir, aiming at providing technical support for the field test of indigenous microorganism enhanced oil recovery. The results showed that bacterial abundance and species in water injection wells were significantly higher than that in no-water injection wells, main bacteria in water injection well were Azospira sp., Pseudomonas sp., Thauera sp.. The species and abundance of archaea in water injection wells also presented differences from no-water injection wells, main archaea were Methanothermobacter thermautotrophicus, Methanosaeta, and Crenarchaeote. Species distribution of bacteria and archaea in water injection wells and no-water injection wells were limited, but their abundances were high, and which were mainly beneficial bacteria increasing oil recovery, indicating that this block possesses the condition to implement the microbial enhanced oil recovery technology by indigenous microorganisms.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第9期83-92,共10页
Biotechnology Bulletin
基金
十一五国家科技重大专项(2011ZX05024-004)
关键词
注水油藏
变性梯度凝胶电泳
细菌
古菌
water injection oil reservoir
denaturing gradient gel electrophoresis
bacteria
archaea
