摘要
生长年限是影响林下山参质量的重要因素之一。为了对不同生长年限的林下山参进行鉴别,该研究采用基于UPLC-Q-TOF/MS的植物代谢组学技术,并结合PCA和OPLS-DA等多变量统计分析方法对11~15年林下山参芦头的代谢物进行研究。结果表明,不同生长年限林下山参芦头之间的代谢物组成有差异。为了提高鉴别效果,通过变量权重重要性排序（VIP）值筛选出对分类贡献大的差异代谢物,并以筛选的差异代谢物建立11~15年林下山参的OPLS-DA判别模型,模型的解释率和预测率均较高,可用于鉴别市场上常见的11~15年林下山参。该研究建立了一种快速、准确、可靠的林下山参年限鉴别方法,同时也为其他中药材的年限鉴别提供了一种新的思路。
Growth year is one of the important factors for the quality of mountain cultivated ginseng（ MCG）. For age differentiation of MCG,rhizome extracts of ginseng aged from 11 to 15 years were analyzed using a non-targeted approach with ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry（ UPLC-Q-TOF/MS）-based on plant metabolomics technique. Multivariate statistical methods such as principal component analysis（ PCA） and orthogonal partial least squared discrimination analysis（ OPLS-DA） were used to compare the derived patterns among the samples. The results showed that the chemical constituents of MCG rhizome extracts of ginseng aged from 11 to 15 years were different. The data set was subsequently applied to metabolite selection by variable importance in the projection（ VIP） for sophisticated classification with the optimal number of metabolites. The OPLS-DA model of MCG has a high interpretability and predictive capability,which established by selecting metabolites of MCG aged from 11 to 15 years. By this approach,MCG samples aged from 11 to 15 years,which are the most in demand in the Chinese ginseng market,can be precisely differentiated on the basis of selected metabolites. This proposed analytical method is fast,accurate,and reliable for discriminating the growth year of MCG. Moreover,this study supplies a new method for the age discrimination of other Chinese medicinal materials.
作者
常相伟
王博然
王彤
李德坤
赵颖
张玉岭
周大铮
叶正良
CHANG Xiang-wei WANG Bo-ran WANG Tong LI De-kun ZHAO Ying ZHANG Yu-ling ZHOU Da-zheng YE Zheng-liang(Tianfin University of Traditional Chinese Medicine, Tianjin 300193, China Tianjin Medical University, Tianjin 300070, China Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300410, China Liaoning Tasly Shenrong Co. , Ltd. , Benxi 117206, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2016年第19期3609-3614,共6页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2013ZX09402202)
