摘要
以10个向日葵品种为试材,利用细胞悬浮技术,研究了培养耐盐细胞系的最佳条件。结果表明:切取向日葵7日龄无菌苗子叶与胚轴接种于6-BA 0.5mg·L^(-1)+NAA 0.5mg·L^(-1)+NaCl 50mmol·L^(-1)+蔗糖40g·L^(-1),pH 5.5~7.0的MS培养基中,培养温度为28℃,14d后取其1g愈伤组织接种于添加NaCl 175mmol·L^(-1)的30mL液体培养基中,振荡培养,7d继代1次,即可获得稳定耐盐细胞系。该方案不仅适合油葵也适用食葵。
Taking 10 varieties of Helianthus annuus L.as test materials,using the cell suspension technology,the optimum conditions for cultivation of salt-tolerant cell lines were studied.The results showed that cutting out the seven-day sunflower′s cotyledons and hypocotyls and inoculating in 6-BA 0.5 mg·L^-1＋ NAA 0.5 mg·L^-1＋ NaCl50mmol·L^-1＋sucrose 40g·L^-1,pH 5.5-7.0 MS medium and culturing in temperature at 28 ℃for two weeks.Then cutting 1g the callus and put them in the 30 mL liquid medium which contained NaCl 175mmol·L^-1,shaking and subculturing every seven days,the stable salt-tolerant cell lines were obtained.This scheme was not only applicable for oil sunflower but also for eating sunflower.
作者
高金秋
宋洋
孙立娟
孙丽娜
张义
GAO Jinqiu SONG Yang SUN Lijuan SUN Li＇na ZHANG Yi(College of Life Sciences, Baicheng Normal University, Baicheng,Jilin 137000 Sunflower Institute, Baieheng Academy of Agricultural Sciences, Baicheng, Jilin 137000)
出处
《北方园艺》
CAS
北大核心
2016年第18期111-114,共4页
Northern Horticulture
基金
吉林省教育厅科学技术研究资助项目(2010-220)
白城师范学院重点资助项目(2014)
关键词
向日葵
细胞悬浮培养
耐盐性
Helianthus annuus L.
cell suspension culture
salt tolerance
