摘要
目的观察灵芝多糖胶囊对免疫抑制小鼠免疫功能的调节作用。方法健康雄性BALB/c小鼠随机分为空白对照组、模型组、香菇多糖组[110mg/(kg.d)]及灵芝多糖低[20mg/(kg.d)]、中[60mg/(kg.d)]和高[180mg/(kg.d)]剂量组,每组10只。每天灌胃给药1次,连续15d,除空白对照组外,其余各组在开始给药后第13天时腹腔注射环磷酰胺80mg/kg,连续注射3d,然后所有组均采用血细胞计数仪观察小鼠外周血细胞数量,称重法观察免疫器官重量指数,MTT法测定刀豆蛋白A(Con A)和脂多糖(LPS)诱导的脾淋巴细胞增殖率,乳酸脱氢酶法检测NK细胞杀伤活性,碳廓清实验观察小鼠碳廓清能力,RT-PCR法检测淋巴细胞白细胞介素-2(IL-2)和γ干扰素(IFN-γ)m RNA表达情况。结果与模型组比较,高剂量灵芝多糖胶囊可提高免疫抑制小鼠外周血WBC数量(P<0.05),中剂量灵芝多糖胶囊可提高免疫抑制小鼠外周血PLT数量(P<0.05)。灵芝多糖低剂量和中剂量组的脾脏指数明显高于模型组(P<0.05),低剂量组的胸腺指数明显高于模型组(P<0.05),低、中、高剂量组Con A和LPS诱导的脾淋巴细胞增殖率、NK细胞杀伤活性及碳廓清指数均明显高于模型组(P<0.01,P<0.01);与模型组比较,低、高剂量灵芝多糖胶囊能够上调脾淋巴细胞中IFN-γ基因的表达(P<0.01),但未观察到灵芝胶囊对脾淋巴细胞IL-2基因表达有上调作用。结论灵芝多糖胶囊对免疫抑制小鼠外周血细胞及免疫功能具有一定保护作用。
Objective To investigate the immune regulation effect of Ganoderma polysaccharide capsule （GPC） on immunosuppressed mice. Methods Sixty mice were randomly divided into 6 groups （10 each）： normal control group, model group, Lentinan group and Z0mg/（kg.d） GPC group, 60mg/（kg·d） GPC group and 180mg/（kg.d） GPC group. The drugs were administrated by intragastric infusion for mice, respectively, once a day for 15 days. From the 13th days, the mice in each group except for control group were injected with cyclophosphamide at a dosage of 80mg/kg, once a day for 3 days. And then the number of peripheral blood cells was counted with veterinary hematology analyzer, the spleen and thymus were weighed and their index were calculated. The T lymphocyte proliferation induced by Con A and LPS was detected with MTT method, NK cytotoxicity was tested with LDH method, carbon clearance was tested by using carbon granules clearance test, and the expressions of IL-2 and IFN-γmRNA in spleen lymphocytes were detected with RT-PCR. Results Peripheral blood WBC in 180mg/（kg·d） GPC group, peripheral blood PLT in 60mg/（kg·d） group, the spleen index in 20mg/（kg·d） and 60mg/（kg.d） groups, and thymus index in 20mg/（kg.d） group were increased remarkably when compared with those in model group （P〈0.05）. Spleen T lymphocyte proliferation induced by Con A and LPS, spleen NK cytotoxicity and carbon clearance in 20mg/（kg·d）, 60mg/（kg·d） and 180mg/（kg·d） GPC groups were increased notably when compared with those in the model group （P〈0.05 or P〈0.01）. The expressions of IFN-γ mRNA in spleen lymphocytes in 20mg/（kg·d） and 180mg/（kg.d） groups increased markedly when compared with that in the model group （P〈0.01）. Conclusion GPS may enhance immune function and increase peripheral blood WBC and PLT in immunosuppressed mice.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2016年第9期719-724,共6页
Medical Journal of Chinese People's Liberation Army
关键词
灵芝属
多糖类
免疫抑制
免疫调节
Ganoderma.polysaccharides
immunosuppression
immunomodulation
