摘要
对从大兴安岭根河地区落叶松和白桦根际土壤中分离的固氮菌进行16S r DNA扩增及序列测定,应用DNAMAN 6.0软件进行多序列比对,用MEGA4软件构建系统发育树。结果表明:大兴安落叶松的根际土壤中分离的40株固氮菌分属于9个不同的类群,其中,有24株菌株属于Burkholderia;白桦根际土壤中分离的19株固氮菌分属于8个不同的类群,有7株菌株属于Burkholderia。表明大兴安岭主要树种兴安落叶松及伴生种白桦根际土壤中固氮菌具有较丰富的多样性,其中Burkholderia为优势种。
The nitrogen - fixing bacteria isolated from the rhizosphere soil of Larix gmelinii and Betula platypylla in Genhe, the Greater Xing＇ an Mountains have been conducted with 16S rDNA amplification and sequence, the mul- tiple sequence alignment has been done by use of DNAMAN 6.0 software, and the phylogenetic tree has been set up with MEGA4 software. The results show 40 strains of nitrogen - fixing bacteria isolated from rhizosphere soil of Larix gmelinii belong to 9 different groups, among them, 24 strains belong to Burkholderia. 24 strains of nitrogen - fixing bacteria isolated from rhizosphere soil of Betula platypylla belong to 8 different groups, among them, 7 strains belong to Burkholderia. The nitrogen - fixing bacteria isolated from the rhizosphere soil of Larix gmelinii and Betula platypylla in the Greater Xing＇ an Mountains are rich in diversity, with Burkholderia as dominant species.
出处
《内蒙古林业科技》
2016年第3期5-9,共5页
Journal of Inner Mongolia Forestry Science and Technology
基金
国家自然科学基金项目(31260173)资助
关键词
落叶松
白桦
固氮菌
16S
RDNA
Larix gmelinii
Betula platypylla
nitrogen - fixing bacteria
16S rDNA
